Difference between revisions of "Part:BBa K567025"
 
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This part is constructed based on PT7-Luc-8AGG ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K567007 BBa_K567007]). The tDNA<sup>Arg</sup> and luciferase gene into T7 operon so that both genes can be transcribed. tRNA<sup>Arg</sup> can be correctly processed and become matured. Luciferase mRNA will be further translated into protein. The induced tRNA<sup>Arg</sup> can facilitate the translation of luciferase-8AGG, achieving positive feedback.  
 
This part is constructed based on PT7-Luc-8AGG ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K567007 BBa_K567007]). The tDNA<sup>Arg</sup> and luciferase gene into T7 operon so that both genes can be transcribed. tRNA<sup>Arg</sup> can be correctly processed and become matured. Luciferase mRNA will be further translated into protein. The induced tRNA<sup>Arg</sup> can facilitate the translation of luciferase-8AGG, achieving positive feedback.  
 
The transcription of this part is under the control of T7 promoter and ''lac'' operator. RNA expression is induced by 0.5mM IPTG when the OD600 of the culture reaches 0.3.
 
The transcription of this part is under the control of T7 promoter and ''lac'' operator. RNA expression is induced by 0.5mM IPTG when the OD600 of the culture reaches 0.3.
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The working curve is shown here.
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[[image:11SJTU_FB8.jpg|center|]]
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=='''Related Biobrick'''==
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PT7-Luc-2AGG-tRNA(Arg)        ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K567022 BBa_K567022])
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PT7-Luc-4AGG-tRNA(Arg)        ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K567023 BBa_K567023])
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PT7-Luc-6AGG-tRNA(Arg)        ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K567024 BBa_K567024])
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PT7-Luc-8AGG-tRNA(Arg)        ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K567025 BBa_K567025])
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Latest revision as of 03:42, 29 October 2011

PT7-Luc-8AGG-tRNA(Arg)

This part is constructed based on PT7-Luc-8AGG (BBa_K567007). The tDNAArg and luciferase gene into T7 operon so that both genes can be transcribed. tRNAArg can be correctly processed and become matured. Luciferase mRNA will be further translated into protein. The induced tRNAArg can facilitate the translation of luciferase-8AGG, achieving positive feedback. The transcription of this part is under the control of T7 promoter and lac operator. RNA expression is induced by 0.5mM IPTG when the OD600 of the culture reaches 0.3.

The working curve is shown here.

11SJTU FB8.jpg

Related Biobrick

PT7-Luc-2AGG-tRNA(Arg) (BBa_K567022)

PT7-Luc-4AGG-tRNA(Arg) (BBa_K567023)

PT7-Luc-6AGG-tRNA(Arg) (BBa_K567024)

PT7-Luc-8AGG-tRNA(Arg) (BBa_K567025)


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 48
    Illegal PstI site found at 1941
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1941
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 48
    Illegal PstI site found at 1941
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 48
    Illegal PstI site found at 1941
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 929