Difference between revisions of "Part:BBa K649100"
Notothenia (Talk | contribs) |
|||
| (5 intermediate revisions by 2 users not shown) | |||
| Line 2: | Line 2: | ||
<partinfo>BBa_K649100 short</partinfo> | <partinfo>BBa_K649100 short</partinfo> | ||
| − | BBa_K649100 contains lsrA promoter and | + | BBa_K649100 contains lsrA promoter and RBS.<br> |
| + | |||
lsrA promoter is repressed by LsrR. In the presense of Phospho-AI2 the transcription of downstream gene is activated. | lsrA promoter is repressed by LsrR. In the presense of Phospho-AI2 the transcription of downstream gene is activated. | ||
| − | To characterize this promoter, we constructed the reporter part BBa_K649104. If you want to see detailed infomation, please click [https://parts.igem.org/Part:BBa_K649104 here.] | + | To characterize this promoter, we constructed the reporter part BBa_K649104. <br> |
| + | [[Image:PlsrA2.png|thumb|center|600px|Fluorescence intensity of BBa_K649104 was much higher than that of promoterless-<i>gfp</i>(negative control).<br>Strain used in this assay lacks <i>lsrR</i>.<br>This work is done by Takuya Tsubaki.]]<br> | ||
| + | |||
| + | If you want to see detailed infomation, please click [https://parts.igem.org/Part:BBa_K649104 here.] | ||
Latest revision as of 14:28, 28 October 2011
LsrA promoter
BBa_K649100 contains lsrA promoter and RBS.
lsrA promoter is repressed by LsrR. In the presense of Phospho-AI2 the transcription of downstream gene is activated.
To characterize this promoter, we constructed the reporter part BBa_K649104.
If you want to see detailed infomation, please click here.
We inproved previous lsrA promoter(BBa_K117002)).our assay of BBa_K117002
For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#5. our work in Tokyo_Tech 2011 wiki].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]