Difference between revisions of "Part:BBa K649400"
Notothenia (Talk | contribs) |
|||
(10 intermediate revisions by 2 users not shown) | |||
Line 2: | Line 2: | ||
<partinfo>BBa_K649400 short</partinfo> | <partinfo>BBa_K649400 short</partinfo> | ||
− | This part contains the | + | This part contains RBS and the isoprene synthase encoding gene(''ispS''). The ispS catalyzes the conversion of dimethylallyl diphoshate to isoprene.<br> |
− | To characterize this part, we constructed the composite part, [https://parts.igem.org/Part:BBa_K649303 BBa_K649303]. BBa_K649303 is composed of PlacIQ(BBa_I14032) and RBS-ispS(BBa_K649400).<br> | + | To characterize this part, we constructed the composite part, [https://parts.igem.org/Part:BBa_K649303 BBa_K649303]. BBa_K649303 is composed of PlacIQ(BBa_I14032) and RBS-''ispS''(BBa_K649400).<br> |
− | + | ||
+ | [[Image:Rain-fig4-2.jpg|thumb|left|400px|isoprene synthesized by ''E. coli'' introduced ''ispS'' gene.<br>This work is done by Yuto Sugiuchi.]] | ||
+ | <div style="clear:both;"></div> | ||
+ | |||
+ | If you are interested in the project related to this parts, please see [http://2011.igem.org/Team:Tokyo_Tech/Projects/making-rain/index.htm our project page in Tokyo_Tech 2011 wiki]. | ||
Latest revision as of 05:17, 29 October 2011
isoprene synthase (ispS)
This part contains RBS and the isoprene synthase encoding gene(ispS). The ispS catalyzes the conversion of dimethylallyl diphoshate to isoprene.
To characterize this part, we constructed the composite part, BBa_K649303. BBa_K649303 is composed of PlacIQ(BBa_I14032) and RBS-ispS(BBa_K649400).
If you are interested in the project related to this parts, please see [http://2011.igem.org/Team:Tokyo_Tech/Projects/making-rain/index.htm our project page in Tokyo_Tech 2011 wiki].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 762
Illegal BglII site found at 1317 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]