Difference between revisions of "Part:BBa K553010:Experience"
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===Applications of BBa_K553010=== | ===Applications of BBa_K553010=== | ||
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| + | UNITS_Trieste Synbiome project | ||
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| + | bacteria Intra-strain communication: | ||
| + | An E. Coli containing BBa_K553010 constitutively produces the OC12 AHL quorum sensing molecule (strain A). The ammount of this molecule is enough to induce the activation of part BBa_K553008 (production of OC8 AHL) in a co-cultivated bacteria (strain B). | ||
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| + | The following assay demonstrates that the organic extract of a co-culture media contains a considerable ammount of OC8. | ||
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| + | [[Image:Coculture_assay.jpg]] | ||
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| + | The diffusion from the center of the OC8 AHL generate, in fonction of its concentration, the activation of a biosensor (A. tumefacines) in Xgal LB agar dishes. | ||
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| + | HPLC-MS/MS assay of an organic extract of strain A with BBa_K553010 in a medium copy number plasmid. | ||
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| + | [[Image:Ms_oc_12.jpg]] | ||
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| + | Blue: culture media 20x concentrated + OC8 and OC12 AHL Standard 1uM - 10 uL loaded | ||
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| + | Red: strain A organic extract 20x concentrated - 10 uL loaded | ||
===User Reviews=== | ===User Reviews=== | ||
Latest revision as of 10:51, 1 November 2011
Datasheet download - experience inside: Media:K553010_lasIcost.pdf
Applications of BBa_K553010
UNITS_Trieste Synbiome project
bacteria Intra-strain communication: An E. Coli containing BBa_K553010 constitutively produces the OC12 AHL quorum sensing molecule (strain A). The ammount of this molecule is enough to induce the activation of part BBa_K553008 (production of OC8 AHL) in a co-cultivated bacteria (strain B).
The following assay demonstrates that the organic extract of a co-culture media contains a considerable ammount of OC8.
The diffusion from the center of the OC8 AHL generate, in fonction of its concentration, the activation of a biosensor (A. tumefacines) in Xgal LB agar dishes.
HPLC-MS/MS assay of an organic extract of strain A with BBa_K553010 in a medium copy number plasmid.
Blue: culture media 20x concentrated + OC8 and OC12 AHL Standard 1uM - 10 uL loaded
Red: strain A organic extract 20x concentrated - 10 uL loaded
User Reviews
UNIQ7c42a7b70865287c-partinfo-00000000-QINU UNIQ7c42a7b70865287c-partinfo-00000001-QINU