Difference between revisions of "Part:BBa K560016"
 
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The backbone for golden gate assembly.  
 
The backbone for golden gate assembly.  
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This backbone is taken from TALEN Golden gate Kit, and it can expedite golden gate cloning together with a set of vectors for golden gate submitted by WHU-igem team. This plasmid contains the coding sequence for α-strand of LacZ. Flanking LacZ are two recognition sites of Bsal, a kind of type IIS restriction endonuclease. If you want to assembly four biobricks at a time, you can mix this backbone with four assembled vectors(<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K560012">BBa_K560012</a>, BBa_K560013, BBa_K560014, BBa_K560015), and add adequate amount of ligase and Bsal.  
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This backbone is taken from TALEN Golden gate Kit, and it can expedite golden gate cloning together with a set of vectors for golden gate submitted by WHU-igem team. This plasmid contains the coding sequence for α-strand of LacZ. Flanking LacZ are two recognition sites of Bsal, a kind of type IIS restriction endonuclease. If you want to assembly four biobricks at a time, you can mix this backbone with four assembled vectors([https://parts.igem.org/wiki/index.php?title=Part:BBa_K560012 BBa_K560012], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K560013 BBa_K560013], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K560014 BBa_K560014], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K560014 BBa_K560015]), and add adequate amount of ligase and Bsal.  
 
More details about experiment protocol can be found in our wiki.  
 
More details about experiment protocol can be found in our wiki.  
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Details about the structure of this plasmid can be found in http://www.addgene.com/31021/.
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Details about the structure of this plasmid can be found in http://www.addgene.org/31021/.
  
  

Latest revision as of 16:38, 14 November 2012

BsaI Golden Gate Assembly_Backbone

The backbone for golden gate assembly. This backbone is taken from TALEN Golden gate Kit, and it can expedite golden gate cloning together with a set of vectors for golden gate submitted by WHU-igem team. This plasmid contains the coding sequence for α-strand of LacZ. Flanking LacZ are two recognition sites of Bsal, a kind of type IIS restriction endonuclease. If you want to assembly four biobricks at a time, you can mix this backbone with four assembled vectors(BBa_K560012, BBa_K560013, BBa_K560014, BBa_K560015), and add adequate amount of ligase and Bsal. More details about experiment protocol can be found in our wiki. Details about the structure of this plasmid can be found in http://www.addgene.org/31021/.


The Genetic Map

PFUS B4-giraffe-analyze-.png


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 644
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 35
    Illegal NheI site found at 2776
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XhoI site found at 363
    Illegal XhoI site found at 1797
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 644
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 644
    Illegal NgoMIV site found at 1650
    Illegal AgeI site found at 569
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 575
    Illegal BsaI.rc site found at 128
    Illegal SapI.rc site found at 2653