Difference between revisions of "Part:BBa K590030"

Latest revision as of 17:57, 26 October 2011

DrR-FabH2-PSB1C3-High constitutive

This part consists of Aldehyde Decarbonylase, from Synechococcus elongatus, ribosomal binding site, Acyl-ACP Reductase, and FabH2 in PSB1C3 high consititutive vector for even chain length alkaneproduction. This construct is designed to produce branched odd chain alkanes. Use of this part results in low alkane yields ( 10mg/L), and results in slow cell growth. FabH2 should be used on a low copy number inducible vector. For more information, refer to [http://2011.igem.org/Team:Washington/Alkanes/Future/FabH2 2011 University of Washington iGEM Team's wiki].

Growth curve showing that cells overexpressing FabH2 are growth deficient. ADC AAR DrR FabH2

This part fails, likely due to toxicity of FabH2. By lowering expression levels and inducing FabH2 production in the FabBrick, we were able to produce even chain length alkanes. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 470
Illegal NheI site found at 493
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1254
Illegal XhoI site found at 2366
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 126
Illegal AgeI site found at 2728
Illegal AgeI site found at 3367
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2773

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K590030 short</partinfo>
-This part consists of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase], from ''Synechococcus elongatus'', ribosomal binding site, [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase], and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590034 FabH2] in PSB1C3 high consititutive vector for even chain length alkaneproduction. This construct is designed to produce branched odd chain alkanes. Use of this part results in low alkane yields ( 10mg/L), and results in slow cell growth. FabH2 should be used on a low copy number inducible vector. For more information, refer to [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team's wiki].
+This part consists of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase], from ''Synechococcus elongatus'', ribosomal binding site, [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase], and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590034 FabH2] in PSB1C3 high consititutive vector for even chain length alkaneproduction. This construct is designed to produce branched odd chain alkanes. Use of this part results in low alkane yields ( 10mg/L), and results in slow cell growth. FabH2 should be used on a low copy number inducible vector. For more information, refer to [http://2011.igem.org/Team:Washington/Alkanes/Future/FabH2 2011 University of Washington iGEM Team's wiki].
+[[Image:Washington_growthcurve.png|center|450px|thumb|Growth curve showing that cells overexpressing FabH2 are growth deficient. [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590026 ADC] [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590027 AAR] [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590030 DrR] [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590030 FabH2]
+]]
 This part fails, likely due to toxicity of FabH2. By lowering expression levels  and inducing FabH2 production in [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590064 the FabBrick], we were able to produce even chain length alkanes.