Difference between revisions of "Part:BBa K560016:Design"
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<partinfo>BBa_K560016 short</partinfo> | <partinfo>BBa_K560016 short</partinfo> | ||
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Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Research, 2011, Vol. 39, No. 12. | Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Research, 2011, Vol. 39, No. 12. | ||
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| + | Details about the structure of this plasmid can be found in http://www.addgene.org/31021/. | ||
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===References=== | ===References=== | ||
Latest revision as of 16:56, 14 November 2012
BsaI Golden Gate Assembly_Backbone
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 644 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal NheI site found at 35
Illegal NheI site found at 2776 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XhoI site found at 363
Illegal XhoI site found at 1797 - 23INCOMPATIBLE WITH RFC[23]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 644 - 25INCOMPATIBLE WITH RFC[25]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal XbaI site found at 644
Illegal NgoMIV site found at 1650
Illegal AgeI site found at 569 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI site found at 575
Illegal BsaI.rc site found at 128
Illegal SapI.rc site found at 2653
Design Notes
N
Source
Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Research, 2011, Vol. 39, No. 12.
Details about the structure of this plasmid can be found in http://www.addgene.org/31021/.