Difference between revisions of "Part:BBa K606027:Experience"
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| − | === | + | ==Characterization: Biobricked YFP:TetR== |
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| + | '''Parts construction''':<br> | ||
| + | <html> | ||
| + | Origins of YFP:TetR are from pFX234 of D. Lane (Toulouse 2 University). | ||
| + | <center><img src="https://static.igem.org/mediawiki/2011/c/cb/YFPtetR10.jpg"> | ||
| + | <p>Cloning plan of YFP:TetR construction</center></p> | ||
| + | </html> | ||
| + | <br><br>We characterize it with TetO Array from pDAG479 plasmid of F-X Barre and D. Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004).<br><br> | ||
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| + | '''Microscopy of double transformed pDAG470 / Biobricked YFP:TetR in <i>E. Coli</i>:''' | ||
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| + | <p>To characterize this part properly, we took pictures of different strains containing YFP:TetR alone and both TetO array (from pDAG479, D.Lane) and expression YFP:TetR system (BBa_K606027).</p> | ||
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| + | <center> | ||
| + | {| border="1" class="wikitable" style="text-align: center;" align="center" | ||
| + | |+ Biobricked YFP:TetR only | ||
| + | |- | ||
| + | |[[Image:yfptetrbb_trans.jpg|350px|thumb|center|Biobricked expression YFP:tetR system in E. coli.]] | ||
| + | |[[Image:yfptetrbb_Fluo2.jpg|350px|thumb|center|Biobricked expression YFP:tetR system in E. coli.]] | ||
| + | |} | ||
| + | {| border="1" class="wikitable" style="text-align: center;" align="center" | ||
| + | |+ Biobricked YFP:TetR and TetO Array | ||
| + | |- | ||
| + | |[[Image:yfptetrbb_teto_trans.jpg|350px|thumb|center|Biobricked expression YFP:tetR system and TetO Array from D. Lane in E. coli.]] | ||
| + | |[[Image:yfptetrbb_teto__Fluo2.jpg|350px|thumb|center|Biobricked expression YFP:tetR system and TetO Array from D. Lane in E. coli.]] | ||
| + | |} | ||
| + | </center> | ||
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| + | The TetR-YFP construct which (emitter part) occasionally shows gross aggregated YFP. <br> | ||
| + | After observing the cells carrying both the TetO and YFP:TetR constructs, we can obviously distinguish glowing dots in some cells. They reflect the behavior we expected. | ||
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| + | More information on : TetO Array characterization (<partinfo>BBa_K606026</partinfo>) and iGEM Paris Bettencourt 2011 wiki [http://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion] | ||
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Latest revision as of 16:27, 24 October 2011
Characterization: Biobricked YFP:TetR
Parts construction:
Origins of YFP:TetR are from pFX234 of D. Lane (Toulouse 2 University).
Cloning plan of YFP:TetR construction
We characterize it with TetO Array from pDAG479 plasmid of F-X Barre and D. Lane (Kinetics of plasmid segregation, Molecular Microbiology, 2004).
Microscopy of double transformed pDAG470 / Biobricked YFP:TetR in E. Coli:
To characterize this part properly, we took pictures of different strains containing YFP:TetR alone and both TetO array (from pDAG479, D.Lane) and expression YFP:TetR system (BBa_K606027).
The TetR-YFP construct which (emitter part) occasionally shows gross aggregated YFP.
After observing the cells carrying both the TetO and YFP:TetR constructs, we can obviously distinguish glowing dots in some cells. They reflect the behavior we expected.
More information on : TetO Array characterization (BBa_K606026) and iGEM Paris Bettencourt 2011 wiki [http://2011.igem.org/Team:Paris_Bettencourt/Experiments/YFP_TetR_diffusion]
User Reviews
UNIQe9a51f0deba255a4-partinfo-00000002-QINU UNIQe9a51f0deba255a4-partinfo-00000003-QINU