Difference between revisions of "Part:BBa K559001:Design"
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Halorhodopsin come from N.pharaonis, gene bank J05199.1 | Halorhodopsin come from N.pharaonis, gene bank J05199.1 | ||
| − | T7 promoter | + | T7 promoter sequence was extracted from pET-23b sequence 303-319 nt, lac operon and RBS was added from the forward primers during PCR amplification on Halorhodopsin. |
| + | (Updated at July 7, 2012) | ||
| − | The above gene is assembled with our halorhodopsin gene <partinfo>BBa_K559000 </partinfo> by 3A assembly, leaving a scar of ' | + | The above gene is assembled with our halorhodopsin gene <partinfo>BBa_K559000 </partinfo> by 3A assembly, leaving a scar of 'tactagag' |
| + | (Updated at July 7, 2012) | ||
===References=== | ===References=== | ||
Latest revision as of 18:39, 7 July 2012
Halorhodopsin under T7 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 368
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 380
Illegal AgeI site found at 106 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 316
Design Notes
The coding sequence of N.pharaonis J05199.1 is selected from 61nt to 700nt and the T7 promotor with lac operon and RBS placed in front of the BBa_K559000 to get constitutive expression of its membrane protein.
Source
Halorhodopsin come from N.pharaonis, gene bank J05199.1
T7 promoter sequence was extracted from pET-23b sequence 303-319 nt, lac operon and RBS was added from the forward primers during PCR amplification on Halorhodopsin. (Updated at July 7, 2012)
The above gene is assembled with our halorhodopsin gene BBa_K559000 by 3A assembly, leaving a scar of 'tactagag' (Updated at July 7, 2012)