Difference between revisions of "Part:BBa K609002:Experience"

Latest revision as of 06:13, 11 October 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K609002

1.In order to test the biobrick, we ligate the part with nisI, which is the part BBa_K609004, and the E.coli-Lactococcus lactis shuttle plasmid pMG36e(the erythromycin resistance gene will be knocked off later). The figures below show the recombinant plasmid and the result of ligation separately.

The restriction pattern of recombinant plasmid for producing collagen.jpg

2.To transduce the recombinant plasmid to S.thermophilus by the means of electroporation.

User Reviews

UNIQdd0b6a1b28eb1e1e-partinfo-00000000-QINU

UNIQdd0b6a1b28eb1e1e-partinfo-00000001-QINU

@@ Line 4: / Line 4: @@
 ===Applications of BBa_K609002===
-<p>1.In order to test the biobrick, we ligate the part with nisI, which is the part BBa_K609004, and pMG36e(for the convenience of screening in E.coli and the erythromycin resistance gene will be knocked off later). The figures below show the recombinant plasmid and the result of ligation separately.</p>
+<p>1.In order to test the biobrick, we ligate the part with ''nisI'', which is the part BBa_K609004, and the ''E.coli-Lactococcus lactis'' shuttle plasmid pMG36e(the erythromycin resistance gene will be knocked off later). The figures below show the recombinant plasmid and the result of ligation separately.</p>
 <center>[[Image:J+N+pMG36e.jpg]]</center>
-<center>'''FIG The recombinant plasmid'''</center>
 <center>[[Image:The_restriction_pattern_of_recombinant_plasmid_for_producing_collagen.jpg‎]]</center>
+<p>2.To transduce the recombinant plasmid to ''S.thermophilus'' by the means of electroporation.
 ===User Reviews===