Difference between revisions of "Part:BBa K676011:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Performed PCR to | + | Performed PCR to clone out the GBS from the pSC101 plasmid. Carried out 2 parts ligation between the cloned GBS and pSB1C3 plasmid backbone. |
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| − | Mark Oram , Alison J. Howells, Anthony Maxwell and Martin L . Pato (2003)A biochemical analysis of the interaction of DNA gyrase with the bacteriophage Mu , pSC101 and pBR322 Strong gyrase Sites ; the role of DNA sequence in modulating gyrase supercoiling and biological activity ; Molecular Microbiology 50(1) | + | Mark Oram, Alison J. Howells, Anthony Maxwell and Martin L . Pato (2003) A biochemical analysis of the interaction of DNA gyrase with the bacteriophage Mu, pSC101 and pBR322 Strong gyrase Sites; the role of DNA sequence in modulating gyrase supercoiling and biological activity; Molecular Microbiology 50 (1) 333-347 |
Latest revision as of 02:20, 10 October 2011
Gyrase Binding Site from pSC101
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Performed PCR to clone out the GBS from the pSC101 plasmid. Carried out 2 parts ligation between the cloned GBS and pSB1C3 plasmid backbone.
Source
pSC101 Plasmid DNA - 4580 to 4864 bp
References
Mark Oram, Alison J. Howells, Anthony Maxwell and Martin L . Pato (2003) A biochemical analysis of the interaction of DNA gyrase with the bacteriophage Mu, pSC101 and pBR322 Strong gyrase Sites; the role of DNA sequence in modulating gyrase supercoiling and biological activity; Molecular Microbiology 50 (1) 333-347