Difference between revisions of "Part:BBa K676011"

Latest revision as of 02:18, 10 October 2011

Gyrase Binding Site from pSC101

The gyrase binding site cloned from pSC101 plasmid.

Usage and Biology

The pSC101 GBS has highly efficient binding to GBS and yet lacks the stimulation of supercoiling (Oram, 2003) and this raises the possibility that the biological effects of gyrase at par are purely structural, for example, local changes in DNA topology due to the wrapping of DNA by gyrase may be already enough to induce the downstream effects of gyrase on pSC101 replication and partition.

Subsequent studies concluded that the key function of par was to cause a local increase in superhelical density in the origin region of pSC101 (Conley and Cohen, 1995), and this in turn was critical in determining the interactions of other replication and partition proteins within the origin itself (Miller and Cohen, 1999). However, further experiments will be necessary to address specific questions relating to the role of gyrase function at par at a more detailed or molecular level.

From the studies, it was also found that the pSC101 GBS is cleaved by gyrase much more efficiently than the pBR322 GBS site, but on the other hand plasmid with pBR322 GBS was supercoiled much better than the one with pSC101 GBS. But still the best supercoiling efficiency was achieved by the plasmid with Mu phage GBS.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K676011 short</partinfo>
-The gyrase site cloned from pSC101 plasmid.
+The gyrase binding site cloned from pSC101 plasmid.
 ===Usage and Biology===
-The pSC101 GBS (highly efficient binding and yet lack of stimulation of
+The pSC101 GBS has highly efficient binding to GBS and yet lacks the stimulation of supercoiling (Oram, 2003) and this raises the possibility that the biological effects of gyrase at par are purely structural, for example, local changes in DNA topology due to the wrapping of DNA by gyrase may be already enough to induce the downstream effects of gyrase on pSC101 replication and partition.
-supercoiling) from  Oram (2003)  raise the possibility that the biological effects of gyrase at par are purely structural, for example , local changes in DNA topology due to the wrapping of DNA by gyrase may be already enough to induce the downstream effects of gyrase on pSC101 replication and partition.
-Subsequent studies concluded that the key function of par was to cause a local increase in
+Subsequent studies concluded that the key function of par was to cause a local increase in superhelical density in the origin region of pSC101 (Conley and Cohen, 1995), and this in turn was critical in determining the interactions of other replication and partition proteins within the origin itself (Miller and Cohen, 1999). However, further experiments will be necessary to address specific questions relating to the role of gyrase function at par at a more detailed or molecular level.
-superhelical density in the origin region of pSC101 (Conley and Cohen, 1995), this in turn was critical in
-determining the interactions of other replication and partition proteins with the origin itself (Miller and Cohen,1999).However ,further experiments will be necessary to address specific questions relating to the role of gyrase function at par at a more detailed or molecular level.
-From the studies, it was also found that  the pSC101 GBS was cleaved  by gyrase much more efficiently than pBR322 site, but plasmid with pBR322 GBS  was supercoiled much better than the one with pSC101. But still the best supercoiling efficiency was achieved by the plasmid with Mu GBS
+From the studies, it was also found that  the pSC101 GBS is cleaved by gyrase much more efficiently than the pBR322 GBS site, but on the other hand plasmid with pBR322 GBS  was supercoiled much better than the one with pSC101 GBS. But still the best supercoiling efficiency was achieved by the plasmid with Mu phage GBS.
 [[Image:Characterising_Part_BBa_K676011.jpg]]
@@ Line 26: / Line 23: @@
 <partinfo>BBa_K676011 parameters</partinfo>
 <!-- -->
-[[Image:Characterising_Part_BBa_K676011.jpg]]