Difference between revisions of "Part:BBa K624032:Experience"
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We measure AMB-1's growth under OD565, which is the best absorption wavelength for AMB-1. For electroporation operation, it is important to find out the log phase, since it’s the best time for electroporation. By keeping AMB-1 in "log phase" (approximately 3~6 days in Enrich-MSGM, 7~10 days in MSGM), best results of electroporation are achieved. And after seven days, the bacterium would go into stationary phase. During the phase, the growth slows as a result of nutrient depletion and accumulation of toxic products. Growth rate and death rate of bacterium is best likely to meet equilibrium in this stage. | We measure AMB-1's growth under OD565, which is the best absorption wavelength for AMB-1. For electroporation operation, it is important to find out the log phase, since it’s the best time for electroporation. By keeping AMB-1 in "log phase" (approximately 3~6 days in Enrich-MSGM, 7~10 days in MSGM), best results of electroporation are achieved. And after seven days, the bacterium would go into stationary phase. During the phase, the growth slows as a result of nutrient depletion and accumulation of toxic products. Growth rate and death rate of bacterium is best likely to meet equilibrium in this stage. | ||
| − | [[image:EX-mms13-SP.jpg|frame|none|PCR from AMB-1 liquid culture. The major band between | + | [[image:EX-mms13-SP.jpg|frame|none|PCR from AMB-1 liquid culture. The major band between 250 bp~500 bp is correct mms13 gene]] |
| + | [[image:mms13 Pmms16 Pmsp3.jpg|frame|none|PCR from AMB-1 liquid culture. We got correct mms13 gene, promoter Pmms16 and promoter Pmsp3 from this PCR. It means we had cultured magnetic bacteria AMB-1 successfully.]] | ||
===User Reviews=== | ===User Reviews=== | ||
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Latest revision as of 15:31, 9 October 2011
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Applications of BBa_K624032
We measure AMB-1's growth under OD565, which is the best absorption wavelength for AMB-1. For electroporation operation, it is important to find out the log phase, since it’s the best time for electroporation. By keeping AMB-1 in "log phase" (approximately 3~6 days in Enrich-MSGM, 7~10 days in MSGM), best results of electroporation are achieved. And after seven days, the bacterium would go into stationary phase. During the phase, the growth slows as a result of nutrient depletion and accumulation of toxic products. Growth rate and death rate of bacterium is best likely to meet equilibrium in this stage.
User Reviews
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