Difference between revisions of "Part:BBa K572100"
 
(4 intermediate revisions by one other user not shown)
Line 1: Line 1:
−
<html>
+
<partinfo>BBa_K572100 short</partinfo>
−
<img src="http://2011.igem.org/File:PSB1PR1.GIF" WIDTH="525PX" HEIGHT="525PX">
+
−
</HTML>
+
  
 
The cloning vector was designed using pSB1C3 backbone. The antibiotic resistance gene is replaced by the proteorhodopsin gene so that the gene gives a metabolic advantage to the transformants on growing in minimal media.
 
The cloning vector was designed using pSB1C3 backbone. The antibiotic resistance gene is replaced by the proteorhodopsin gene so that the gene gives a metabolic advantage to the transformants on growing in minimal media.
 +
 +
<!-- Add more about the biology of this part here
 +
===Usage and Biology===
 +
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K572100 SequenceAndFeatures</partinfo>
 +
 +
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K572100 parameters</partinfo>
 +
<!-- -->
 +
<html>
 +
<img src="https://static.igem.org/mediawiki/parts/d/de/PSB1Pc.png" WIDTH="843PX" HEIGHT="648PX">
 +
</HTML>

Latest revision as of 01:51, 29 October 2011

Light based screening plasmid backbone - pSB1Pc

The cloning vector was designed using pSB1C3 backbone. The antibiotic resistance gene is replaced by the proteorhodopsin gene so that the gene gives a metabolic advantage to the transformants on growing in minimal media.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2139
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2145
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2139
    Illegal XhoI site found at 1033
    Illegal XhoI site found at 2015
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2139
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2139
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2154
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.