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| | __NOTOC__ | | __NOTOC__ |
| − | =iGEM11_OUC Experience= | + | ===Applications of BBa_K594015 === |
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| | ===User Reviews=== | | ===User Reviews=== |
| − | <!-- DON'T DELETE --><partinfo>BBa_K594015 StartReviews</partinfo>
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| − | {|width='80%' style='border:1px solid gray'
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| − | <partinfo>BBa_K594015 AddReview 5</partinfo>
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| − | <I>DonQuixia</I>
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| − | |width='60%' valign='top'|
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| − | When we use the pSB1C3 for standardization, we found that the pSB1C3 have the right antibiotic resistance but a wrong DNA length.
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| − | <!-- DON'T DELETE --><partinfo>BBa_K594015 EndReviews</partinfo>
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| − | ===The verification===
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| − | [[Image:OUC-China.Review.jpg|center]]
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| − | The pSB1C3 is supposed to be 2049bp, however, it appears around the marker 3000bp.
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| − | We have the pSB1C3 in K381001 sequenced and found its total length is 2944bp, and its total sequence is as below,
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| − | [[Image:OUC-China.ExpDNA.png|center]]
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| − | Through sequence analysis, we could find that the base in blue have a 99% homology with the right pSB1C3. It lacks a C and a A in the beginning. In the end, it has a 71 base homology with the right pSB1C3. The other sequence left do not accord with the right pSB1C3.
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