|
|
| (8 intermediate revisions by the same user not shown) |
| Line 1: |
Line 1: |
| | __NOTOC__ | | __NOTOC__ |
| − | == OUC China Experience==
| + | ===Applications of BBa_K594015 === |
| − | ===Applications of BBa_K594015=== | + | |
| − | From the beginning, we use the linearized plasmid backbone pSB1C3 in the 2011 spring DNA distribution. But the standardization with them all end up with failures.
| + | |
| | | | |
| − | Then, we began to seek for a new plasmid backbone. We found the K381001 in pSB1C3. If we cut the pSB1C3 with EcoR I and Pst I, we could get pSB1C3 plasmid backbone for standardization.
| |
| | | | |
| | ===User Reviews=== | | ===User Reviews=== |
| − | <!-- DON'T DELETE --><partinfo>BBa_K594015 StartReviews</partinfo>
| |
| − | {|width='80%' style='border:1px solid gray'
| |
| − | |-
| |
| − | |width='10%'|
| |
| − | <partinfo>BBa_K594015 AddReview 5</partinfo>
| |
| − | <I>DonQuixia</I>
| |
| − | |width='60%' valign='top'|
| |
| − | When we use the pSB1C3 for standardization, we found that the pSB1C3 have the right antibiotic resistance but a wrong DNA length.
| |
| − | |}
| |
| − | <!-- DON'T DELETE --><partinfo>BBa_K594015 EndReviews</partinfo>
| |
| − | ===The verification===
| |
| − | [[Image:OUC-China.Review.jpg|center]]
| |
| − | The pSB1C3 is supposed to be 2049bp, however, it appears around the marker 3000bp.
| |
| − | We have the pSB1C3 in K381001 sequenced and found its total length is 2944bp, and its total sequence is as below,
| |
| − | <html>
| |
| − | <html><style type="text/css">ol{margin:0;padding:0}p{margin:0}.c4{width:468pt;background-color:#ffffff;padding:90pt 72pt 90pt 72pt}.c3{text-align:justify;direction:ltr}.c2{color:#0000ff}.c1{color:#f50000}.c0{font-size:10pt}body{color:#000000;font-size:12pt;font-family:Times New Roman}h1{padding-top:12pt;line-height:1.0;text-align:left;color:#000000;font-size:16pt;font-family:Arial;font-weight:bold;padding-bottom:3pt}h2{padding-top:12pt;line-height:1.0;text-align:left;color:#000000;font-style:italic;font-size:14pt;font-family:Arial;font-weight:bold;padding-bottom:3pt}h3{padding-top:12pt;line-height:1.0;text-align:left;color:#000000;font-size:13pt;font-family:Arial;font-weight:bold;padding-bottom:3pt}h4{padding-top:12pt;line-height:1.0;text-align:left;color:#000000;font-size:14pt;font-family:Times New Roman;font-weight:bold;padding-bottom:3pt}h5{padding-top:12pt;line-height:1.0;text-align:left;color:#000000;font-style:italic;font-size:13pt;font-family:Times New Roman;font-weight:bold;padding-bottom:3pt}h6{padding-top:12pt;line-height:1.0;text-align:left;color:#000000;font-size:11pt;font-family:Times New Roman;font-weight:bold;padding-bottom:3pt}</style><div class="c4"><p class="c3"><span class="c0">GTGGAGTT</span><span class="c2 c0">CACCCTG</span><span class="c0 c1">C(lack a C)</span><span class="c2 c0">CCTTTTTCTTT</span><span class="c1 c0">A(lack a A)</span></p><p class="c3"><span class="c0 c2">AAACCGAAAAGATTACTTCGCGTTATGCAGGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCACAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGCTCGAGGCTTGGATTCTCACCAATAAAAAACGCCCGGCGGCAACCGAGCGTTCTGAACAAATCCAGATGGAGTTCTGAGGTCATTACTGGATCTATCAACAGGAGTCCAAGCGAGCTCGATATCAAATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTGAATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGAAATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGATAACTCAAAAAATACGCCCGGTAGTGATCTTATTTCATTATGGTGAAAGTTGGAACCTCTTACGTGCCCGATCAACTCGAGT</span><span class="c0">TACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAA</span><span class="c1 c0">AGTGCCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTATCACGAGGC</span><span class="c0">GCA</span></p></div>
| |
| − | </html>
| |
| − | Through sequence analysis, we could find that the base in blue have a 99% homology with the right pSB1C3. It lacks a C and a A in the beginning. In the end, it has a 71 base homology with the right pSB1C3. The other sequence left do not accord with the right pSB1C3.
| |
