Difference between revisions of "Part:BBa K624054"
 
(One intermediate revision by the same user not shown)
(No difference)

Latest revision as of 23:18, 5 October 2011

mcherry+minC+RBS+tetO+Pmsp1 + Pmsp1+RBS+tetR+GFP

mcherry+minC+RBS+tetO+Pmsp1 + Pmsp1+RBS+tetR+GFP

This part is composed of BBa_K624045 and BBa_K624055, both of two are submitted by 2011 NYMU-Taipei iGEM Team. The former subpart is in reverse order. We chose not to assembly BBa_K624045 in the downstream of BBa_K624055. If so, unwanted leakiness may happen. The construct was inserted into pYMB(BBa_K624027) and then was electropored into AMB-1(BBa_K624032).

AMB-1 minC NYMU.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 842
    Illegal PstI site found at 1622
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 842
    Illegal PstI site found at 1622
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 842
    Illegal PstI site found at 1622
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 842
    Illegal PstI site found at 1622
    Illegal NgoMIV site found at 2599
    Illegal AgeI site found at 681
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4262