Difference between revisions of "Part:BBa K624060"
Phonesents (Talk | contribs) |
|||
(2 intermediate revisions by the same user not shown) | |||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K624060 short</partinfo> | <partinfo>BBa_K624060 short</partinfo> | ||
− | pYMB essentials + RBS(Pmsp3, 6 bps | + | pYMB essentials + RBS(trunc.) + minC |
+ | |||
+ | pYMB ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624004 BBa_K624004]) is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone pSB1A1 with promoter Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria. | ||
+ | |||
+ | The ribosome binding site is from Psmp3 with 6 bps truncated.([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624013 BBa_K624013]). | ||
+ | |||
+ | MinC ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624033 BBa_K624033]) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites. | ||
+ | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 11:56, 9 October 2011
pYMB essentials + RBS(trunc.) + minC
pYMB essentials + RBS(trunc.) + minC
pYMB (BBa_K624004) is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone pSB1A1 with promoter Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria.
The ribosome binding site is from Psmp3 with 6 bps truncated.(BBa_K624013).
MinC (BBa_K624033) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 898
Illegal AgeI site found at 1389 - 1000COMPATIBLE WITH RFC[1000]