Difference between revisions of "Part:BBa K649300"
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Original rocF has two EcoRI sites in ORF, so we changed these sites. | Original rocF has two EcoRI sites in ORF, so we changed these sites. | ||
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To characterize this part, we constructed the composite part(BBa_K649301). To know detailed infomation, please click [https://parts.igem.org/Part:BBa_K649301 here]. | To characterize this part, we constructed the composite part(BBa_K649301). To know detailed infomation, please click [https://parts.igem.org/Part:BBa_K649301 here]. | ||
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| + | [[Image:RocF_on_3K3_MG_only.png|thumb|center|400px|Urea concentration in growth media 1 hour after IPTG induction.<br /> | ||
| + | This work is done by Natsuki Kubo.]] | ||
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| + | Introduction of BBa_K649301 containing arginase coding gene (rocF) led to production of some urea in strain MG1655 (argR +). | ||
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Latest revision as of 14:12, 28 October 2011
Arginase (rocF)
This part has only rocF obtained from B.subtilis 168 by colony-PCR.
rocF is the cording region of Arginase(EC:3.5.3.1), catalyzing the reaction from arginine and water to urea and ornithine.
Original rocF has two EcoRI sites in ORF, so we changed these sites.
To characterize this part, we constructed the composite part(BBa_K649301). To know detailed infomation, please click here.
Introduction of BBa_K649301 containing arginase coding gene (rocF) led to production of some urea in strain MG1655 (argR +).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 137
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]