Difference between revisions of "Part:BBa K624052"

Latest revision as of 12:46, 9 October 2011

Pmsp1(tetO) + RBS(trunc.) + minC + RBS(trunc.) + mcherry

Pmsp1+RBS(trunc.)+tetR+RBS(trunc.)+GFP+Ter regulates this construct by producing tet repressor in the presence of tetracycline.

This construct expresses in pYMB. pYMB (BBa_K624004) is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone pSB1A1 with promoter Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria.

All of the ribosome binding sites are RBS from Pmsp3 with 6 bps truncated(BBa_K624013).

MinC (BBa_K624033) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 445
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K624052 short</partinfo>
-pYMB essentials + RBS(Pmsp3, 6 pbs trunc.) + minC + mcherry
+Pmsp1(tetO) + RBS(trunc.) + minC + RBS(trunc.) + mcherry
+Pmsp1+RBS(trunc.)+tetR+RBS(trunc.)+GFP+Ter regulates this construct by producing tet repressor in the presence of tetracycline.
+This construct expresses in pYMB. pYMB ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624004 BBa_K624004]) is described to contain the ori (origin of replication), rep gene (required for replication) of pMGT. Once the synthetic work had been done, pYMB is constructed by equipping the ori, the appropriate promoter for AMB-1 (Pmms16 and Pmsp3 as our candidate) and rep gene on the commercial plasmid pUG19 which was revised as the expression of Biobrick backbone pSB1A1 with promoter Pmsp1. The constructed vector is capable of replicating within both E. coli and AMB-1, fully sufficing a competent shuttle vector for genetic engineering the magnetotactic bateria.
+All of the ribosome binding sites are RBS from Pmsp3 with 6 bps truncated([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624013 BBa_K624013]).
+MinC ([https://parts.igem.org/wiki/index.php?title=Part:BBa_K624033 BBa_K624033]) is known as a cell division inhibitor. Studies show that minC interacts directly with FtsZ and antagonizes FtsZ assembly. Overexpression of minC would lead to septation inhibition at all potential division sites.
 <!-- Add more about the biology of this part here