Difference between revisions of "Part:BBa K544003"
 
(2 intermediate revisions by 2 users not shown)
Line 11: Line 11:
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K544003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K544003 SequenceAndFeatures</partinfo>
 +
 +
  
 
<div ALIGN=CENTER>
 
<div ALIGN=CENTER>
Line 17: Line 19:
 
  |[[Image:Table 1.jpg|450px]]
 
  |[[Image:Table 1.jpg|450px]]
 
  |-
 
  |-
−
  |Our team leader is demonstrating the pipetting techniques to the high school students.
+
  |Fluorescent intensity of MG1655 -tetO2-0-sfGFP strain after transformation of various repressors
 
  |}
 
  |}
 
</div>
 
</div>
  
  
−
Fluorescent intensity of MG1655 -tetO2-0-sfGFP strain after transformation of various repressors
+
 
 
(The data collected are considered only when difference between values with the P-value <0.05)
 
(The data collected are considered only when difference between values with the P-value <0.05)
 +
 +
  
 
<div ALIGN=CENTER>
 
<div ALIGN=CENTER>
Line 30: Line 34:
 
  |[[Image:Table 2.jpg|450px]]
 
  |[[Image:Table 2.jpg|450px]]
 
  |-
 
  |-
−
  |Our team leader is demonstrating the pipetting techniques to the high school students.
+
  |Fluorescent intensity of MG1655 -tetO2-0-EGFP strain after transformation of various repressors
 
  |}
 
  |}
 
</div>
 
</div>
  
  
−
Fluorescent intensity of MG1655 -tetO2-0-EGFP strain after transformation of various repressors
 
 
(The data collected are considered only when difference between values with the P-value <0.05)
 
(The data collected are considered only when difference between values with the P-value <0.05)
 +
 
[EGFP: Enhanced Green Fluorescent Protein, sfGFP: Superfolder Green Fluorescent protein ]
 
[EGFP: Enhanced Green Fluorescent Protein, sfGFP: Superfolder Green Fluorescent protein ]
  

Latest revision as of 19:23, 5 October 2011

Tetracycline Repressor with Consitutive Promoter BBa_J23116

A transcriptional regulator TetR (BBa_C0040) is driven by constitutive promoter family members (BBa-J23103, BBa-J23109, BBa-J23116, and BBa-J23106), which can be used to tune the expression level of constitutively expressed TetR.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 65
    Illegal SpeI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 37
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 65
    Illegal SpeI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 65
    Illegal SpeI site found at 37
  • 1000
    COMPATIBLE WITH RFC[1000]


Table 1.jpg
Fluorescent intensity of MG1655 -tetO2-0-sfGFP strain after transformation of various repressors


(The data collected are considered only when difference between values with the P-value <0.05)


Table 2.jpg
Fluorescent intensity of MG1655 -tetO2-0-EGFP strain after transformation of various repressors


(The data collected are considered only when difference between values with the P-value <0.05)

[EGFP: Enhanced Green Fluorescent Protein, sfGFP: Superfolder Green Fluorescent protein ]