Difference between revisions of "Part:BBa K543012"
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[[Image:BBa_K543012.jpg|right|200px]] | [[Image:BBa_K543012.jpg|right|200px]] | ||
| − | '''Qualitative assay''' | + | '''Qualitative assay'''<br> |
Our experience is that inoculated E.coli (induced K543012) onto a plate. Then put filter paper on center of plate and spot 20ul of culture AHL producing E.coli in. After 37℃ incubation, check florescence of GFP.<br> | Our experience is that inoculated E.coli (induced K543012) onto a plate. Then put filter paper on center of plate and spot 20ul of culture AHL producing E.coli in. After 37℃ incubation, check florescence of GFP.<br> | ||
Figure is result of assay. GFP is expressed around of filter paper. This result suggest that K543012 is work appropriately. <br> | Figure is result of assay. GFP is expressed around of filter paper. This result suggest that K543012 is work appropriately. <br> | ||
Latest revision as of 18:35, 5 October 2011
plux-GFP (AHL inducible)
This device is useful for detective of AHL(3OC6HSL). Under existence of AHL, GFP is expressed.
Qualitative assay
Our experience is that inoculated E.coli (induced K543012) onto a plate. Then put filter paper on center of plate and spot 20ul of culture AHL producing E.coli in. After 37℃ incubation, check florescence of GFP.
Figure is result of assay. GFP is expressed around of filter paper. This result suggest that K543012 is work appropriately.
K543012 is twin with J55000 and I733005, but both of them are only planning, haven’t experienced yet. Our result is the first data of this device is work.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 985
Illegal BsaI.rc site found at 1713