Difference between revisions of "Part:BBa K649301"
 
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Arginase is coded with Ptrc and RBS in this part.<br />
 
Arginase is coded with Ptrc and RBS in this part.<br />
  
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rocF is the same as BBa_K649300.<br />
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<i>rocF</i> is the same as [https://parts.igem.org/Part:BBa_K649300 BBa_K649300].<br />
 
Ptrc and RBS are from pTrc99A.<br />
 
Ptrc and RBS are from pTrc99A.<br />
  
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In our test, when this part was ligated into pSB1C3, <span style="font-style:italic;">E. coli</span> DH5&alpha; didn't glow even after 24 hours incubation.<br />
 
In our test, when this part was ligated into pSB1C3, <span style="font-style:italic;">E. coli</span> DH5&alpha; didn't glow even after 24 hours incubation.<br />
  
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[[Image:BBa K649301 graph(2).png|thumb|center|500px|Urea concentration in growth media 1 hour after IPTG induction.<br />
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[[Image:RocF_on_3K3_MG_only.png|thumb|center|400px|Urea concentration in growth media 1 hour after IPTG induction.<br />
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This work is done by Natsuki Kubo.]]
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This work was done by Natsuki Kubo.]]
  
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Introduction of BBa_K649301 containing arginase coding gene (<span style="font-style:italic;">rocF</span>) led to production of some urea in strain MG1655 (<span style="font-style:italic;">argR</span> +). This part produced urea more efficiently in strain JD24293 (<span style="font-style:italic;">argR</span> -).<br /><br />
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Introduction of [https://parts.igem.org/Part:BBa_K649301 BBa_K649301] containing arginase coding gene (<span style="font-style:italic;">rocF</span>) led to production of some urea in strain MG1655.<br /><br />
 
For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/method our work in Tokyo_Tech 2011 wiki.]
 
For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/method our work in Tokyo_Tech 2011 wiki.]
  

Latest revision as of 05:03, 29 October 2011

Ptrc-RBS-rocF

Arginase is coded with Ptrc and RBS in this part.

rocF is the same as BBa_K649300.
Ptrc and RBS are from pTrc99A.

You should NOT ligate this part into high-copy vector.
In our test, when this part was ligated into pSB1C3, E. coli DH5α didn't glow even after 24 hours incubation.

Urea concentration in growth media 1 hour after IPTG induction.
This work was done by Natsuki Kubo.

Introduction of BBa_K649301 containing arginase coding gene (rocF) led to production of some urea in strain MG1655.

For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/method our work in Tokyo_Tech 2011 wiki.]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 230
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]