Difference between revisions of "Part:BBa K581009"
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===Design Notes=== | ===Design Notes=== | ||
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===References=== | ===References=== | ||
| − | * [http://nar.oxfordjournals.org/content/30/10/e43.full.pdf+html]. | + | * [http://nar.oxfordjournals.org/content/30/10/e43.full.pdf+html] David M. Hoover, Jacek Lubkowski. DNAWorks: an automated method for designing oligonucleotides for PCR-based gene synthesis. ''Nucl. Acids Res'' 30 (10): e4 (2002). |
Latest revision as of 16:12, 5 October 2011
pqrr-GFP ssrA degradation tag
GFP behind promoter qrr
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 936
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 936
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 936
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 936
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 831
Design Notes
▪ promoter qrr was amplified by DNAWorks
References
- [http://nar.oxfordjournals.org/content/30/10/e43.full.pdf+html] David M. Hoover, Jacek Lubkowski. DNAWorks: an automated method for designing oligonucleotides for PCR-based gene synthesis. Nucl. Acids Res 30 (10): e4 (2002).