Difference between revisions of "Part:BBa K543015"

 
(15 intermediate revisions by the same user not shown)
Line 4: Line 4:
 
TetR generator (J23100-B0031-R0040-B0015).
 
TetR generator (J23100-B0031-R0040-B0015).
  
 +
[[Image:Tokyo_Metropolitan_Killing2.jpg|right|250px]]
 +
'''Repress expression of GFP by Anti-killing device'''<br>
 +
 +
We confirmed whether Anti-killing Device is working or not. We transform Anti-killing Device into E.coli which have GFP generator(I13522) and observed its florescence. Figure is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. we confirmed Anti-killing Device represses GFP expression weakly.<br>
 +
Need more experience to get quantitative date. <br>
  
<font size="3"><b>Repress expression of GFP by Anti-killing device</b></font><br>
 
<table>
 
<tr><td width="500">
 
We confirm whether Anti-killing Device is working or not. We transform Anti-killing Device into E.coli which have GFP generator(I13522:Ptet-B0034-GFP-B0015) and observe its florescence. Figure2 is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. Although not perfectly repressed GFP expression, we confirmed Anti-killing Device is working. Figure5 is difference of GFP florescence by comparing with absorbance of 525nm.
 
For perfectly repressed expression cording under Ptet, RBS is needed to be more strong(ex.B0034).In addition, we should do more experience to get quantitative date.
 
  
</td><td width="300"><img src="https://static.igem.org/mediawiki/2011/c/cd/Tokyo_Metropolitan_Killing2.png" width="300"><br><div align="center">Figure5: repress expression of GFP by Anti-killing device</div></td></tr>
 
</table>
 
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
quantitative research
 
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 16:07, 5 October 2011

TetR generator (J23100-B0031-R0040-B0015)

TetR generator (J23100-B0031-R0040-B0015).

Tokyo Metropolitan Killing2.jpg

Repress expression of GFP by Anti-killing device

We confirmed whether Anti-killing Device is working or not. We transform Anti-killing Device into E.coli which have GFP generator(I13522) and observed its florescence. Figure is a result of this experiment. Left is E.coli transformed Anti-killing Device, right is control. we confirmed Anti-killing Device represses GFP expression weakly.
Need more experience to get quantitative date.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]