Difference between revisions of "Part:BBa K524001"
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The split sfGFP1-10 was constructed from an pBAD driven sfGFP [https://parts.igem.org/Part:BBa_I746908 BBa_I746908]. CDS of sfGFP includes amino acid residues 1-214 of sfGFP.
 
The split sfGFP1-10 was constructed from an pBAD driven sfGFP [https://parts.igem.org/Part:BBa_I746908 BBa_I746908]. CDS of sfGFP includes amino acid residues 1-214 of sfGFP.
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Split sfGFPs at amino acid residues 214 and 215 have been reported to undergo spontaneous complementation to give green fluorescence.
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The amino acid residues 214 and 215 of sfGFP have been reported to undergo spontaneous complementation to restore green fluorescence. Hence when this biobrick is expressed together with [https://parts.igem.org/Part:BBa_K524002 BBa_K524002], which contains the the remaining 215-238 amino acids of sfGFP (sfGFP11), the resulting proteins should be able to complement and fluoresce.
  
 
===Usage and Biology===
 
===Usage and Biology===
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The split sfGFP1-10 in this biobrick is assembled behind R0010 Lac promoter.
 
  
 
Induced by IPTG, the product of this biobrick should complement with the product of IPTG inducible biobrick sfGFP11 [https://parts.igem.org/Part:BBa_K524002 BBa_K524002] to give green fluorescence under UV.
 
Induced by IPTG, the product of this biobrick should complement with the product of IPTG inducible biobrick sfGFP11 [https://parts.igem.org/Part:BBa_K524002 BBa_K524002] to give green fluorescence under UV.

Latest revision as of 15:59, 5 October 2011

pLac + RBS + split sfGFP 1-10

The split sfGFP1-10 was constructed from an pBAD driven sfGFP BBa_I746908. CDS of sfGFP includes amino acid residues 1-214 of sfGFP. The amino acid residues 214 and 215 of sfGFP have been reported to undergo spontaneous complementation to restore green fluorescence. Hence when this biobrick is expressed together with BBa_K524002, which contains the the remaining 215-238 amino acids of sfGFP (sfGFP11), the resulting proteins should be able to complement and fluoresce.

Usage and Biology

Induced by IPTG, the product of this biobrick should complement with the product of IPTG inducible biobrick sfGFP11 BBa_K524002 to give green fluorescence under UV.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 238