Difference between revisions of "Part:BBa K524003:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | The <i>nadE< | + | The <i>nadE</i> gene in the <i>E. coli</i> genome is originally terminated by downstream extragenic sites. The extragenic sties were replaced with the double terminator [https://parts.igem.org/Part:BBa_B0015 BBa_B0015] in our construct. |
===Source=== | ===Source=== | ||
− | The nadE gene was cloned out from the genome of E. coli strain BL21(DE3). | + | The <i>nadE</i> gene was cloned out from the genome of <i>E. coli</i> strain BL21(DE3). |
===References=== | ===References=== | ||
− | Ecocyc: Encyclopedia of Escherichia coli K-12 Genes and Metabolism | + | Ecocyc: Encyclopedia of <i>Escherichia coli</i> K-12 Genes and Metabolism |
Latest revision as of 15:43, 5 October 2011
nadE gene
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The nadE gene in the E. coli genome is originally terminated by downstream extragenic sites. The extragenic sties were replaced with the double terminator BBa_B0015 in our construct.
Source
The nadE gene was cloned out from the genome of E. coli strain BL21(DE3).
References
Ecocyc: Encyclopedia of Escherichia coli K-12 Genes and Metabolism