Difference between revisions of "Part:BBa K649301"

Latest revision as of 05:03, 29 October 2011

Ptrc-RBS-rocF

Arginase is coded with Ptrc and RBS in this part.

rocF is the same as BBa_K649300.
Ptrc and RBS are from pTrc99A.

You should NOT ligate this part into high-copy vector.
In our test, when this part was ligated into pSB1C3, E. coli DH5α didn't glow even after 24 hours incubation.

Urea concentration in growth media 1 hour after IPTG induction.
This work was done by Natsuki Kubo.

Introduction of BBa_K649301 containing arginase coding gene (rocF) led to production of some urea in strain MG1655.

For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/method our work in Tokyo_Tech 2011 wiki.]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 230
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 Arginase is coded with Ptrc and RBS in this part.<br />
-rocF is the same as BBa_K649300.<br />
+<i>rocF</i> is the same as [https://parts.igem.org/Part:BBa_K649300 BBa_K649300].<br />
 Ptrc and RBS are from pTrc99A.<br />
@@ Line 10: / Line 10: @@
 In our test, when this part was ligated into pSB1C3, <span style="font-style:italic;">E. coli</span> DH5&alpha; didn't glow even after 24 hours incubation.<br />
-[[Image:BBa K649301 graph(2).png|thumb|center|500px|Urea concentration in growth media 1 hour after IPTG induction.<br />
+[[Image:RocF_on_3K3_MG_only.png|thumb|center|400px|Urea concentration in growth media 1 hour after IPTG induction.<br />
-This work is done by Natsuki Kubo.]]
+This work was done by Natsuki Kubo.]]
-Introduction of BBa_K649301 containing arginase coding gene (<span style="font-style:italic;">rocF</span>) led to production of some urea in strain MG1655 (<span style="font-style:italic;">argR</span> +). This part produced urea more efficiently in strain JD24293 (<span style="font-style:italic;">argR</span> -).<br /><br />
+Introduction of [https://parts.igem.org/Part:BBa_K649301 BBa_K649301] containing arginase coding gene (<span style="font-style:italic;">rocF</span>) led to production of some urea in strain MG1655.<br /><br />
-For more information, see[http://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/index.htm. our work in Tokyo_Tech 2011 wiki.]
+For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/Urea-cooler/method our work in Tokyo_Tech 2011 wiki.]
 <!-- Add more about the biology of this part here