Difference between revisions of "Part:BBa K598007"
 
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This is a GFP generator with a theophylline responsive riboswitch sequence. The mRNA with a theophylline riboswitch in it would respond to theophylline concerntration, producing different amount of GFP measured by GFP fluorescence intensity. Under the control of different promoters, it could be used to demonstrate the theophylline responsive curve, providing data for modeling. The theophylline riboswitch sequence could also be used to modulate other downstream gene expression.
 
This is a GFP generator with a theophylline responsive riboswitch sequence. The mRNA with a theophylline riboswitch in it would respond to theophylline concerntration, producing different amount of GFP measured by GFP fluorescence intensity. Under the control of different promoters, it could be used to demonstrate the theophylline responsive curve, providing data for modeling. The theophylline riboswitch sequence could also be used to modulate other downstream gene expression.
 
In this part, the theophylline responsive riboswitch contains an original ribosome binding site.
 
In this part, the theophylline responsive riboswitch contains an original ribosome binding site.
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===Usage and Biology===
 
===Usage and Biology===
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<partinfo>BBa_K598007 parameters</partinfo>
 
<partinfo>BBa_K598007 parameters</partinfo>
 
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<p><b>For the full characterisation of the device, please refer to [https://parts.igem.org/wiki/index.php?title=Part:BBa_K598005 BBa_K598005]

Latest revision as of 09:29, 5 October 2011

Theophylline Responsive Riboswitch P1G1 with Native RBS+E0040+B0015 This is a GFP generator with a theophylline responsive riboswitch sequence. The mRNA with a theophylline riboswitch in it would respond to theophylline concerntration, producing different amount of GFP measured by GFP fluorescence intensity. Under the control of different promoters, it could be used to demonstrate the theophylline responsive curve, providing data for modeling. The theophylline riboswitch sequence could also be used to modulate other downstream gene expression. In this part, the theophylline responsive riboswitch contains an original ribosome binding site.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 731


For the full characterisation of the device, please refer to BBa_K598005