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| − | ===Construction of BBa_K567013===
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| − | This biobrick is constructed first by cloning the tRNA<sup>Asp</sup> from ''AspV'' in ''E.coli'', then the anticodon region is site-directed mutated. This part is constructed on the backbone plasmid pACYC184.
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| − | ===Characterization of BBa_K567013===
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| − | This part acts as a stop codon suppressor tRNA. It can be charged with Asp.
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| − | We have used P''bla''-Luc-TAG as our Reporter. The amount of luciferase produced is reflected using the bioluminescence emitted during the luciferin reaction. Our results demonstrate that this part can suppress TAG insertion into luciferase. In the experimental group, with the help of BBa_K567011 PT7-TDRS (Favorite Part), luciferase-TAG was produced and bioluminescence was emitted during the luciferin reaction. '''These results proved that this tRNA<sup>Asp</sup> can effectively suppress stop codon.
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| − | [[image:11SJTU-stop-codon_switch.jpg|frame|center|fig. Functional Analysis of Stop-Codon Switch. ER2566 cannot produce luciferase with BBa_K567003 Pbla-Luc-TAG only. When BBa_K567011 PT7-TDRS (Favorite Part) and BBa_K567013 tRNA<sup>Asp</sup>-TAG (Favorite Part) are also transformed into the cell, luciferase is produced. The results proved Stop-Codon Switch as a strict molecular switch without background noise.This tRNA<sup>Asp</sup> is part of the Stop-Codon Switch]]
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| | ===User Reviews=== | | ===User Reviews=== |
Latest revision as of 10:38, 5 October 2011
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UNIQ174a538255d458bb-partinfo-00000000-QINU
UNIQ174a538255d458bb-partinfo-00000001-QINU
