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| | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter |
| | how you used this part and how it worked out. | | how you used this part and how it worked out. |
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| − | ===Construction of BBa_K567016===
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| − | ''metY''-CGA: We have cloned operon ''metY'' containing tRNA<sup>Met</sup> from ''E.coli'' to pACYC184. The anticodon of tRNA<sup>Met</sup> was mutated to TCG (base pairing codon CGA).
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| − | ===Characterization of BBa_K567016===
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| − | When this part, MetRS PT7-''metG''N (BBa_K567015)(or MetRS PT7-''metG''M (BBa_K567014)) and KanaR (BBa_K567020) are co-transformed into the cell, the cell is expected to survive Kana.
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| − | [[image:11SJTU-initial_codon_result.jpg|frame|center|fig. Growth of ER2566 with a. ''metG''N + ''metY''-CGA, b. ''metG''M + ''metY''-CGA, c. + ''metG''N, d. + ''metG''M. Growth medium (left): LB Kana+Tet. Growth medium (right): LB Kana.]]
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| − | Cell growth shows that the cells show Kana resistance only when both modified MetRS (''metG''N) and modified tRNA<sup>Met</sup>(''metY''-CGA) are transformed into the cell, proving that ''metG''N works well.
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| − | For more information concerning this part, please see [http://2011.igem.org/Team:SJTU-BioX-Shanghai 2011 SJTU-BioX-iGEM]
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| | ===User Reviews=== | | ===User Reviews=== |
Latest revision as of 10:36, 5 October 2011
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