Difference between revisions of "Part:BBa K598017:Design"
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===References=== | ===References=== | ||
| + | Maumita Mandal & Ronald R Breaker.(2004)Adenine riboswitches and gene activation by disruption of a transcription terminator. Nature structural& molecular biology | ||
| + | Jörg S. Hartig et al. (2008) Artificial Ribozyme Switches Containing Natural Riboswitch Aptamer Domains. Angew. Chem. 121: 2753 –2756 | ||
Latest revision as of 06:21, 5 October 2011
pBAD+Adenine Hammerhead Ribozyme+RBS+E0040+B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 919
Design Notes
This part constructs by connecting adenine aptamer from add riboswitch with stem III of wildtype hammerhead ribozyme by a selected linker of twelve basepairs.
Source
The adenine aptamer was first found in V. vulnificus and the fast-cleaving hammerhead ribozyme is from Schistosoma mansonii
References
Maumita Mandal & Ronald R Breaker.(2004)Adenine riboswitches and gene activation by disruption of a transcription terminator. Nature structural& molecular biology Jörg S. Hartig et al. (2008) Artificial Ribozyme Switches Containing Natural Riboswitch Aptamer Domains. Angew. Chem. 121: 2753 –2756