Difference between revisions of "Part:BBa K602008"

 
 
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<partinfo>BBa_K602008 short</partinfo>
 
<partinfo>BBa_K602008 short</partinfo>
  
 
<i>D. radiodurans</i> RecA with LacI constitutive promoter and ribosome binding site attached upstream. Constitutively expresses RecA. Should increase DNA damage repair capability and radioresistance of host <i>E. coli</i>.
 
<i>D. radiodurans</i> RecA with LacI constitutive promoter and ribosome binding site attached upstream. Constitutively expresses RecA. Should increase DNA damage repair capability and radioresistance of host <i>E. coli</i>.
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'''''Please see Experience page for characterization details.'''''
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<partinfo>BBa_K602008 parameters</partinfo>
 
<partinfo>BBa_K602008 parameters</partinfo>
 
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=='''Characterization'''==
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[https://2019.igem.org/Team:Botchan_Lab_Tokyo Team Botchan_Lab_Tokyo 2019] contributed to characterization of this part by testing growth ratio of <i>E.coli</i>.<br> (<small>--[[User:youma| youma]] 21:21, 21. October 2019 (UTC) </small>)
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=='''Team Botchan_Lab_Tokyo 2019 characterization'''==
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We characterized BBa_K602008.
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=='''description'''==
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It is RecA (composite parts) that combined the psB1C3 is vector in the basic parts with RecA is the coding parts in an in-fusion cloning. The graph below shows the growth curves of competent cells, DH5α without this plasmid and the composite parts.
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[[image:T--Botchan_Lab_Tokyo--recAgraph.pdf|600px|thumb|center]]
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Fig1. growth curve
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This chart is standard deviation (SD) of the technical replicate, and the SD is calculated based on data that originally follow a normal distribution. Therefore, it is necessary to determine whether the data follow a normal distribution by the Shapiro-Wilk test. However, the SD was calculated assuming that all data follow a normal distribution because the number of samples worthy of the Shapiro Wilk test cannot be shake-cultured due to laboratory problems.
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=='''conclusion'''==
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Competent cells grew as expected.

Latest revision as of 02:14, 22 October 2019

D. radiodurans RecA expression device

D. radiodurans RecA with LacI constitutive promoter and ribosome binding site attached upstream. Constitutively expresses RecA. Should increase DNA damage repair capability and radioresistance of host E. coli.

Please see Experience page for characterization details.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 450
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1124
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Team Botchan_Lab_Tokyo 2019 contributed to characterization of this part by testing growth ratio of E.coli.
(-- youma 21:21, 21. October 2019 (UTC) )

Team Botchan_Lab_Tokyo 2019 characterization

We characterized BBa_K602008.

description

It is RecA (composite parts) that combined the psB1C3 is vector in the basic parts with RecA is the coding parts in an in-fusion cloning. The graph below shows the growth curves of competent cells, DH5α without this plasmid and the composite parts.

T--Botchan Lab Tokyo--recAgraph.pdf

Fig1. growth curve

This chart is standard deviation (SD) of the technical replicate, and the SD is calculated based on data that originally follow a normal distribution. Therefore, it is necessary to determine whether the data follow a normal distribution by the Shapiro-Wilk test. However, the SD was calculated assuming that all data follow a normal distribution because the number of samples worthy of the Shapiro Wilk test cannot be shake-cultured due to laboratory problems.

conclusion

Competent cells grew as expected.