Difference between revisions of "Part:BBa K658020"
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This part is made up of two subparts, a population-control device iccdB0.6 (BBa_K658003) and an EGFP (BBa_J04630) reporter. The population-control device iccdB0.6 (BBa_K658003) has the ability to maintain steady-state cell density at a relatively low value and extend the steady state in cell growth. The reporter EGFP’s (BBa_J04630) expression is regulated by the iccdB0.6 in upstream. | This part is made up of two subparts, a population-control device iccdB0.6 (BBa_K658003) and an EGFP (BBa_J04630) reporter. The population-control device iccdB0.6 (BBa_K658003) has the ability to maintain steady-state cell density at a relatively low value and extend the steady state in cell growth. The reporter EGFP’s (BBa_J04630) expression is regulated by the iccdB0.6 in upstream. | ||
− | + | [[Image:XMU_China_36.jpg|left|Figure 1 Proposed mechanism of iccdB0.6-GFP|frame|Figure 1 Proposed mechanism of iccdB0.6-GFP.]] | |
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+ | <img src="https://static.igem.org/mediawiki/parts/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
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== '''Description''' == | == '''Description''' == | ||
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== '''Result''' == | == '''Result''' == | ||
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− | Figure 2 Experimentally measured fluorescence at steady state of BL21's cells with iccdB0.6-GFP(BBa_K658020) and BBa_K658021. | + | [[Image:XMU_China_37.jpg|left|Figure 2 Experimentally measured fluorescence at steady state of BL21's cells with iccdB0.6-GFP(BBa_K658020) and BBa_K658021|frame|Figure 2 Experimentally measured fluorescence at steady state of BL21's cells with iccdB0.6-GFP(BBa_K658020) and BBa_K658021.]] |
+ | <html> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/4/41/XMU_China_block.jpg"> | ||
+ | </html> | ||
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== '''Reference''' == | == '''Reference''' == | ||
− | [1] Koch B, Liljefors T, Persson T , Nielsen J, Kjelleberg S, Givskov M. The LuxR receptor: the sites of interaction with quorum-sensing signals and inhibitors[J].Microbiology , 2005, 151 (11): 3589-3602. | + | [1]You L, Cox RS, Weiss R, Arnold FH. Programmed population control by cell-cell communication and regulated killing[J]. Nature, 2004, 428(6985): 868-871. |
+ | |||
+ | [2]Koch B, Liljefors T, Persson T , Nielsen J, Kjelleberg S, Givskov M. The LuxR receptor: the sites of interaction with quorum-sensing signals and inhibitors[J].Microbiology , 2005, 151 (11): 3589-3602. | ||
− | [ | + | [3] Kumari A, Pasini P, Deo S. K., Flomenhoft D, Shashidhar S, Daunert S. Biosensing Systems for the Detection of Bacterial Quorum Signaling Molecules[J]. Analytical Chemistry,2005, 78 (22): 7603–7609. |
− | [ | + | [4] Eberhard A, Burlingame A. L, Eberhard C, Kenyon G. L, Nealson K. H,Oppenheimer N. J. Structural identification of autoinducer of Photobacterium fischeri luciferase[J]. Biochemistry ,1981,20 (9): 2444–2449. |
− | [ | + | [5] http://scholarworks.umass.edu/dissertations/AAI3397713/ |
− | [ | + | [6] Gerdes K. Toxin-antitoxin modules may regulate synthesis of macromolecules during nutritional stress[J]. J. Bacteriol,2000,182 (3): 561–721. |
− | [ | + | [7]Philippe B,Martine C.Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes[J].Molecular Biology,1992,226(3):735-745. |
− | [ | + | [8]Devine J.H,Shadel G.S,Baldwin T.O. Identification of the operator of the lux regulon from the Vibrio fischeri strain ATCC7744. Proceedings of the National Academy of Sciences.1989.86(15):5688-5692. |
− | [ | + | [9] http://www.uniprot.org/uniprot/P12747 |
− | [ | + | [10]Miki T,Yoshioka K,Horiuchi T. Control of cell division by sex factor F in Escherichia coli. I. The 42.84-43.6 F segment couples cell division of the host bacteria with replication of plasmid DNA. Molecular Biology,1984,177(4):605-625. |
Latest revision as of 08:22, 15 October 2011
iccdB->GFP
This device is designed to test how a population-control device could influence the expression of its downstream genes.
This part is made up of two subparts, a population-control device iccdB0.6 (BBa_K658003) and an EGFP (BBa_J04630) reporter. The population-control device iccdB0.6 (BBa_K658003) has the ability to maintain steady-state cell density at a relatively low value and extend the steady state in cell growth. The reporter EGFP’s (BBa_J04630) expression is regulated by the iccdB0.6 in upstream.
Description
This device is an example of how population-control device could be used in regulating the expression of our desired product, such as antibiotics and proteins.
Result
Reference
[1]You L, Cox RS, Weiss R, Arnold FH. Programmed population control by cell-cell communication and regulated killing[J]. Nature, 2004, 428(6985): 868-871.
[2]Koch B, Liljefors T, Persson T , Nielsen J, Kjelleberg S, Givskov M. The LuxR receptor: the sites of interaction with quorum-sensing signals and inhibitors[J].Microbiology , 2005, 151 (11): 3589-3602.
[3] Kumari A, Pasini P, Deo S. K., Flomenhoft D, Shashidhar S, Daunert S. Biosensing Systems for the Detection of Bacterial Quorum Signaling Molecules[J]. Analytical Chemistry,2005, 78 (22): 7603–7609.
[4] Eberhard A, Burlingame A. L, Eberhard C, Kenyon G. L, Nealson K. H,Oppenheimer N. J. Structural identification of autoinducer of Photobacterium fischeri luciferase[J]. Biochemistry ,1981,20 (9): 2444–2449.
[5] http://scholarworks.umass.edu/dissertations/AAI3397713/
[6] Gerdes K. Toxin-antitoxin modules may regulate synthesis of macromolecules during nutritional stress[J]. J. Bacteriol,2000,182 (3): 561–721.
[7]Philippe B,Martine C.Cell killing by the F plasmid CcdB protein involves poisoning of DNA-topoisomerase II complexes[J].Molecular Biology,1992,226(3):735-745.
[8]Devine J.H,Shadel G.S,Baldwin T.O. Identification of the operator of the lux regulon from the Vibrio fischeri strain ATCC7744. Proceedings of the National Academy of Sciences.1989.86(15):5688-5692.
[9] http://www.uniprot.org/uniprot/P12747
[10]Miki T,Yoshioka K,Horiuchi T. Control of cell division by sex factor F in Escherichia coli. I. The 42.84-43.6 F segment couples cell division of the host bacteria with replication of plasmid DNA. Molecular Biology,1984,177(4):605-625.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 718
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2347
Illegal BsaI.rc site found at 1874
Illegal BsaI.rc site found at 3310