Difference between revisions of "Part:BBa K649001"

Latest revision as of 02:47, 14 October 2011

GFP regulated by 3OC12-HSL and LasR

Fluorescence intensity of BBa_K649001 was increased by 3OC12-HSL induction. GFP expression after induction of 3OC12-HSL is 170 times as high as before.

Effect of 3OC12-HSL induction on fluorescence intensity
This work is done by Takuya Tsubaki.

Generally, in the presence of 3OC12-HSL, LasR activates lasI promoter and the transcription level of downstream gene increases, but in the absence of 3OC12-HSL, LasR can't activate lasI promoter. 2011 iGEM Tokyo-Tech characterized BBa_K649000 by means of using BBa_K649001 composed of BBa_K649000 and BBa_J54103.

We improved previous las promoters.

For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#3. our work in Tokyo_Tech 2011 wiki].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 118
Illegal BamHI site found at 106
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 805

Revision as of 04:36, 5 October 2011 (view source) Notothenia (Talk \| contribs) ← Older edit		Latest revision as of 02:47, 14 October 2011 (view source) Notothenia (Talk \| contribs)
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−	Fluorescence intensity of BBa_K649001 was increased by 3OC12-HSL induction.	+	Fluorescence intensity of BBa_K649001 was increased by 3OC12-HSL induction. GFP expression after induction of 3OC12-HSL is 170 times as high as before.