Difference between revisions of "Part:BBa K649000"
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<partinfo>BBa_K649000 short</partinfo>
 
<partinfo>BBa_K649000 short</partinfo>
  
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In the presence of 3OC12HSL lasR can bind to the operator of this promoter and activate the transcription of downstream gene, whereas in the absence of 3OC12HSL lasR can't bind to the operator and can't activate the transcription.
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In the presence of 3OC12-HSL lasR can bind to the operator of this promoter and activate the transcription of downstream gene, whereas in the absence of 3OC12-HSL lasR can't bind to the operator and can't activate the transcription.
  
  
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To characterize this promoter, we constructed the reporter part composed of BBa_K649000 and BBa_J54103. If you want to see detailed infomation, please click [https://parts.igem.org/Part:BBa_K649001 here.]
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To characterize this promoter, we constructed the reporter part composed of BBa_K649000 and BBa_J54103. To know detailed infomation, please click [https://parts.igem.org/Part:BBa_K649001 here].
  
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[[Image:BBa K649001 graph3.png|thumb|center|500px|Effect of 3OC12-HSL induction on fluorescence intensity<br>This work is done by Takuya Tsubaki.]]
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GFP expression after induction of 3OC12-HSL is 170 times as high as before.
  
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We improved previous lasI promoter(BBa_J64010).[https://parts.igem.org/Part:BBa_J64010:Experience our assay of BBa_J64010]
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We improved previous las promoters.<br>
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<ol>
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<li>
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For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#3. our work in Tokyo_Tech 2011 wiki]  
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[https://parts.igem.org/Part:BBa_J64010:Experience our assay of BBa_J64010]
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</li>
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<li>
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[https://parts.igem.org/Part:BBa_K091117 BBa_K091117]
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</li>
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<li>
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[https://parts.igem.org/Part:BBa_R0079 BBa_R0079]
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</li>
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</ol>
  
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K649000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K649000 SequenceAndFeatures</partinfo>
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==Contribution of SCAU-China 2023==
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'''What have we done? '''
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<p>We introduced this part to achieve control over drug concentration through bacterial density. We successfully characterized its performance in ''E. coli'' TOP10. For more detailed characterization results, please refer to our composite_part-'''Part:BBa_K4632024''' [https://parts.igem.org/Part:BBa_K4632024].</p>
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Latest revision as of 23:15, 10 October 2023

LasR and 3OC12HSL regulated promoter.

In the presence of 3OC12-HSL lasR can bind to the operator of this promoter and activate the transcription of downstream gene, whereas in the absence of 3OC12-HSL lasR can't bind to the operator and can't activate the transcription.


To characterize this promoter, we constructed the reporter part composed of BBa_K649000 and BBa_J54103. To know detailed infomation, please click here.

Effect of 3OC12-HSL induction on fluorescence intensity
This work is done by Takuya Tsubaki.

GFP expression after induction of 3OC12-HSL is 170 times as high as before.

We improved previous las promoters.

  1. our assay of BBa_J64010
  2. BBa_K091117
  3. BBa_R0079


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Contribution of SCAU-China 2023

What have we done?

We introduced this part to achieve control over drug concentration through bacterial density. We successfully characterized its performance in E. coli TOP10. For more detailed characterization results, please refer to our composite_part-Part:BBa_K4632024 [1].