Difference between revisions of "Part:BBa K137018:Experience"
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<I>XMU-China 2011</I> | <I>XMU-China 2011</I> | ||
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| − | Lux R is expressed upon induction by isopropyl-b-D-thiogalactopyranoside (IPTG).The acyl-homoserine lactone (AHL) signaling molecule can bind and | + | Lux R is expressed upon induction by isopropyl-b-D-thiogalactopyranoside (IPTG).The acyl-homoserine lactone (AHL) signaling molecule can bind and activate the Lux R transcriptional regulator, which induces the expression of GFP under the control of lux pR. |
The figure below shows the performance of E.coli strain BL21 with the biobrick K137018 when exposed to ultraviolet light. IPTG and AHL are added to the medium. The concentrations of the signaling molecules(AHL) are 0, 2, 4, 6, 8, 10nmol, respectively. | The figure below shows the performance of E.coli strain BL21 with the biobrick K137018 when exposed to ultraviolet light. IPTG and AHL are added to the medium. The concentrations of the signaling molecules(AHL) are 0, 2, 4, 6, 8, 10nmol, respectively. | ||
| − | + | [[Image:XMU_China_k137018f1.jpg|left]] | |
| + | <html> | ||
| + | <img src="https://static.igem.org/mediawiki/parts/thumb/4/41/XMU_China_block.jpg/600px-XMU_China_block.jpg"> | ||
| + | </html> | ||
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From the figure, we can see that the higher the concentration of the signaling molecules(AHL), the stronger the fluorescence intensity. | From the figure, we can see that the higher the concentration of the signaling molecules(AHL), the stronger the fluorescence intensity. | ||
| + | ===important parameters=== | ||
| + | [[Image:XMU_China_k137018f2.jpg|left]] | ||
|}; | |}; | ||
Latest revision as of 13:05, 5 October 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K137018
This part is meant to measure the response of the part F2622 to induction with acylhomoserine lactone (AHL). By measuring GFP flourescence on a flow cytometer, the ability of F2622 to activate transcription of a target gene can be measured.
User Reviews
UNIQ5f0373e4e7c19579-partinfo-00000000-QINU UNIQ5f0373e4e7c19579-partinfo-00000001-QINU
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XMU-China 2011 |
Lux R is expressed upon induction by isopropyl-b-D-thiogalactopyranoside (IPTG).The acyl-homoserine lactone (AHL) signaling molecule can bind and activate the Lux R transcriptional regulator, which induces the expression of GFP under the control of lux pR. The figure below shows the performance of E.coli strain BL21 with the biobrick K137018 when exposed to ultraviolet light. IPTG and AHL are added to the medium. The concentrations of the signaling molecules(AHL) are 0, 2, 4, 6, 8, 10nmol, respectively. 
important parameters |
