Difference between revisions of "Part:BBa K649200"

Latest revision as of 05:10, 29 October 2011

PlacIQ-lox2272-gfp-lox2272

lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise. The Cre-mediated recombination of this BioBrick had been studied by in vitro assay, and proved to be working.

in vitro assay of K649200
negative control, 0.5hr, 2hr, and 4hr from left

For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#7.1. our work in Tokyo_Tech 2011 wiki].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 107
Illegal BamHI site found at 13
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 790

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K649200 short</partinfo>
-lox2272 is a mutant of loxP which have mutations in the 8 bp spacer region. DNA segment flanked by two lox2272 marks the point which the enzyme Cre will excise.
+''lox2272'' is a mutant of ''loxP'' which have mutations in the 8 bp spacer region. DNA segment flanked by two ''lox2272'' marks the point which the enzyme Cre will excise.
 The Cre-mediated recombination of this BioBrick had been studied by ''in vitro'' assay, and proved to be working.
+[[Image:in vitro_lox_gfp_lox.png|thumb|center|500px|<br/>|''in vitro'' assay of K649200<br />negative control, 0.5hr, 2hr, and 4hr from left]]
+For more information, see [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#7.1. our work in Tokyo_Tech 2011 wiki].
 <!-- Add more about the biology of this part here