Difference between revisions of "Part:BBa K646000"

Latest revision as of 16:18, 10 May 2013

Hemeoxygenase with RBS

Synechocystis hemeoxygenase with a Ribosome Binding Site (RBS), oxidises Heme into biliverdin which absorbs light in the 360 nm and 650 nm wavelength producing a green colouration.

Usage and Biology

The BioBrick part in team Macquarie University (2011) mechanism

When coupled to a promoter BioBrick such as T7 promoter (BBa_I719005 and BBa_I712074) to produce parts BBa_K646003 and BBa_K646004, and transformed into Bl21(DE3)Tuner E. coli cells, a green colouration (require induction with ALA and IPTG) is produced.

Liquid media containing IPTG and ALA, incubated for 24 hours at low temperature, spun down. Pellets are green due to the production of biliverdin from the breakdown of heme - this indicates that heme oxyganse was expressed using T7 promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-When coupled to a promoter BioBrick such as T7 promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_I719005 BBa_I719005] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074]), and transformed into Bl21(DE3)Tuner ''E. coli'' cells, a green colouration is produced.
+[[Image:MQ_New_data_page_diagram.jpg|center|750px]] <br> '''The BioBrick part in team Macquarie University (2011) mechanism'''<br><br>
+When coupled to a promoter BioBrick such as T7 promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_I719005 BBa_I719005] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074]) to produce parts [https://parts.igem.org/wiki/index.php?title=Part:BBa_K646003 BBa_K646003] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K646004 BBa_K646004], and transformed into Bl21(DE3)Tuner ''E. coli'' cells, a green colouration (require induction with ALA and IPTG) is produced.
 [[Image:Results_-_green colonies.JPG|400px]]
-Liquid media containing IPTG and ALA, incubated for 24 hours at low temperature, spun down. Pellets are green as heme oxyganse was expressed using T7 promoter and degraded heme into biliverdin.
+Liquid media containing IPTG and ALA, incubated for 24 hours at low temperature, spun down. Pellets are green due to the production of biliverdin from the breakdown of heme - this indicates that heme oxyganse was expressed using T7 promoter.