Difference between revisions of "Part:BBa K572009"
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− | .. | + | It is an oxidoreductase-enzyme which catalyzes the conversion of beta-carotene to retinal in the presence of oxygen, according to the reaction: |
+ | <br>beta-carotene + O2 <=> 2 retinal.<br/> | ||
+ | The product of this enzymatic reaction, i.e Retinal is a VitaminA aldehyde. It is a polyene chromophore which binds to proteins called 'Opsins' (say Proteorhodopsin). This retinal-opsin complex allows microorganisms to convert light energy to metabolic energy. | ||
+ | Hence in micro-organisms functionally expressing Proteorhodopsin, beta-carotene dioxygenase converts beta-carotene to Trans-retinal, which is required for the functioning of Proteorhodopsin. | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
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+ | <img src=" https://static.igem.org/mediawiki/parts/a/a0/K572009_Page_1.jpg" width="750px" height="971px" /> | ||
+ | <img src="https://static.igem.org/mediawiki/parts/e/ee/K572009_Page_2.jpg" width="750px" height="971px" /> | ||
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Latest revision as of 23:31, 28 October 2011
Mouse Beta Carotene Dioxygenase
It is an oxidoreductase-enzyme which catalyzes the conversion of beta-carotene to retinal in the presence of oxygen, according to the reaction:
beta-carotene + O2 <=> 2 retinal.
The product of this enzymatic reaction, i.e Retinal is a VitaminA aldehyde. It is a polyene chromophore which binds to proteins called 'Opsins' (say Proteorhodopsin). This retinal-opsin complex allows microorganisms to convert light energy to metabolic energy.
Hence in micro-organisms functionally expressing Proteorhodopsin, beta-carotene dioxygenase converts beta-carotene to Trans-retinal, which is required for the functioning of Proteorhodopsin.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 82
Illegal PstI site found at 220 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 82
Illegal PstI site found at 220 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1443
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 82
Illegal PstI site found at 220 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 82
Illegal PstI site found at 220 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 418
Illegal SapI site found at 999