Difference between revisions of "Part:BBa K524004"
 
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<partinfo>BBa_K524004 short</partinfo>
 
<partinfo>BBa_K524004 short</partinfo>
  
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The pir gene codes for the pi protein. Initiation of replication at ori-gamma requires the pi protein in trans. Doubling the concentration of pi protein would, however, effectively shut down the replication. Expression of pi protein is autogenously regulated.  
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The pir gene encodes the π protein. Initiation of replication at ori-γ or other R6K origins of replication requires the π protein in trans. Increasing its expression to double the natural concentration produced would, however, effectively shut down the replication. Expression of π protein is autogenously regulated.  
  
 
===Usage and Biology===
 
===Usage and Biology===
  
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The presence of pir gene can allow maintenance of plasmids with R6K origins as their sole origin of replication.
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The presence of pir gene allow maintenance of plasmids with R6K origins as their sole origin of replication.
  
 
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Latest revision as of 15:48, 5 October 2011

pir gene

The pir gene encodes the π protein. Initiation of replication at ori-γ or other R6K origins of replication requires the π protein in trans. Increasing its expression to double the natural concentration produced would, however, effectively shut down the replication. Expression of π protein is autogenously regulated.

Usage and Biology

The presence of pir gene allow maintenance of plasmids with R6K origins as their sole origin of replication.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 438
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]