Difference between revisions of "Part:BBa K574009:Design"

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===Design Notes===
 
===Design Notes===
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We want to design a system whose input is 3OC12HSL, then we combine LasR coding sequence(''<partinfo>C0079</partinfo>'') with relevant promoter(''<partinfo>R0079</partinfo>''). Considering the possibility for various designs in the future(or in other words, the expandability), those regulated by Plas promoter depends on your need.
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We want to design a system whose input is 3OC12HSL, thus we combine LasR coding sequence(''<partinfo>C0079</partinfo>'') with relevant promoter(''<partinfo>R0079</partinfo>''). Considering the possibility for various designs in the future(or in other words, the expandability), those regulated by pLas promoter depend on your need.
  
 
===Source===
 
===Source===

Latest revision as of 14:56, 3 October 2011

3OC12HSL -> PoPS Receiver


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1048
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 386
    Illegal AgeI site found at 583
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We want to design a system whose input is 3OC12HSL, thus we combine LasR coding sequence(BBa_C0079) with relevant promoter(BBa_R0079). Considering the possibility for various designs in the future(or in other words, the expandability), those regulated by pLas promoter depend on your need.

Source

We used BioBrick Standard Assembly. List as follows: BBa_K081005 BBa_C0079 BBa_B0015 BBa_R0079

References