Difference between revisions of "Part:BBa K598010:Design"

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===Design Notes===
 
===Design Notes===
 +
 +
This part is designed to test the performance of ligand responsive RNA controller. GFP is used as reporter gene.
 +
Change the promoter, terminater, reporter gene, or RNA controller if necessary.
 
Check the RBS sequence before using.
 
Check the RBS sequence before using.
−
 
−
 
  
 
===Source===
 
===Source===
  
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By PCR.
+
BBa_I13453, BBa_E0040, and BBa_B0015 come from the constitutive library.
 +
RNA controller sequence is inserted between promoter and AUG initiation codon via PCR and bluntend ligation.
  
 
===References===
 
===References===
 
Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614.
 
Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614.

Latest revision as of 15:26, 4 October 2011

pBAD+Theophylline Responsive Riboswitch 1G1 with Engineered RBS+E0040+B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
    Illegal XhoI site found at 209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 868


Design Notes

This part is designed to test the performance of ligand responsive RNA controller. GFP is used as reporter gene. Change the promoter, terminater, reporter gene, or RNA controller if necessary. Check the RBS sequence before using.

Source

BBa_I13453, BBa_E0040, and BBa_B0015 come from the constitutive library. RNA controller sequence is inserted between promoter and AUG initiation codon via PCR and bluntend ligation.

References

Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614.