Difference between revisions of "Part:BBa K598009:Design"

(→‎Source)
(→‎Source)
 
(One intermediate revision by the same user not shown)
Line 6: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
Change the promoter if required.
+
This part is designed to test the performance of ligand responsive RNA controller. GFP is used as reporter gene.
−
 
+
  
 +
Change the promoter, terminater, reporter gene, or RNA controller if necessary.
  
 
===Source===
 
===Source===
  
−
BBa_I13453, BBa_E0040, and BBa_B0015 come from the constitutive promoter library.
+
BBa_I13453, BBa_E0040, and BBa_B0015 come from the constitutive library.
 
RNA controller sequence is inserted between promoter and AUG initiation codon via PCR and bluntend ligation.
 
RNA controller sequence is inserted between promoter and AUG initiation codon via PCR and bluntend ligation.
  
 
===References===
 
===References===
 
Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614.
 
Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614.

Latest revision as of 15:14, 4 October 2011

pBAD+Theophylline Responsive Riboswitch P1G1 with Native RBS+E0040+B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 869


Design Notes

This part is designed to test the performance of ligand responsive RNA controller. GFP is used as reporter gene.

Change the promoter, terminater, reporter gene, or RNA controller if necessary.

Source

BBa_I13453, BBa_E0040, and BBa_B0015 come from the constitutive library. RNA controller sequence is inserted between promoter and AUG initiation codon via PCR and bluntend ligation.

References

Beatrix Suess, Barbara Fink, Christian Berens, Régis Stentz and Wolfgang Hillen. (2004).A theophylline responsive riboswitch based on helix slipping controls gene expression in vivo. Nucleic Acids Research 32, 1610-1614.