Difference between revisions of "Part:BBa K557000:Design"

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We obtained the sequence of CheZ of Top10 in NCBI (NP416395.1) and designed primers to acquire CheZ by PCR.
 
We obtained the sequence of CheZ of Top10 in NCBI (NP416395.1) and designed primers to acquire CheZ by PCR.
 
   
 
   
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forward primer:5'-GTTTCGAATTCGCGGCCGCTTCTAGATGCAACCATCAATCAAACCTGCTGACGAGCAT-3'
 
  
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reverse primer:5'-GTTTCCTGCAGCGGCCGCTACTAGTA-3'
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<p>forward primer:5'-GTTTCGAATTCGCGGCCGCTTCTAGATGCAACCATCAATCAAACC-3'</p>
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<p>reverse primer:5'-GTTTCCTGCAGCGGCCGCTACTAGTATTATTAAAATCCAAGTCTATCCAACAAATCGT-3'</p>
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===Source===
 
===Source===

Latest revision as of 13:21, 2 October 2011

CheZ: phosphatase which dephosphorylates CheY-P and thus plays an important role in E.Coli chemotaxi


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We obtained the sequence of CheZ of Top10 in NCBI (NP416395.1) and designed primers to acquire CheZ by PCR.


forward primer:5'-GTTTCGAATTCGCGGCCGCTTCTAGATGCAACCATCAATCAAACC-3'

reverse primer:5'-GTTTCCTGCAGCGGCCGCTACTAGTATTATTAAAATCCAAGTCTATCCAACAAATCGT-3'

Source

Escherichia coli, the strain has always been kept in the lab of USTC-IGEM

References

Shana Topp, Justin P. Gallivan (2006) Guiding Bacteria with Small Molecules and RNA. J.Am.Chem.Soc, 129,6870-6811