Difference between revisions of "Part:BBa K624017"

Latest revision as of 12:29, 9 October 2011

pYMB essentials+[Y-13-R]+[CHAMP]

pYMB indicates pUC19 with ori+Pmsp1+rep. In this construct, Y-13-R and CHAMP are ligated in the downstream of ori+Pmsp1+rep, aimed at serving as optogenetic wireless neurostimulator with OFF state (no BRET between EYFP and rLuc) as default mode.

We design this construct with the whole YFP on the N-terminus of Mms13 and the CHAMP design to induce the BRET phenomenon. We suppose that with the CHMAP design, the construct will light up only when magnetic force is applied.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 898
Illegal NgoMIV site found at 2073
Illegal NgoMIV site found at 2157
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2823

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 __NOTOC__
 <partinfo>BBa_K624017 short</partinfo>
-pYMB indicates pUC19 with ori+Pmsp1+rep. In this construct, Y-13-R and CHAMP is ligated in the downstream of ori+Pmsp1+rep, aimed at serving as optogenetic wireless neurostimulator with OFF state (no BRET between EYFP and rLuc) as default mode.
+pYMB indicates pUC19 with ori+Pmsp1+rep. In this construct, Y-13-R and CHAMP are ligated in the downstream of ori+Pmsp1+rep, aimed at serving as optogenetic wireless neurostimulator with OFF state (no BRET between EYFP and rLuc) as default mode.
+[[Image: c3_NYMU.png|frame|none|We design this construct with the whole YFP on the N-terminus of Mms13 and the CHAMP design to induce the BRET phenomenon. We suppose that with the CHMAP design, the construct will light up only when magnetic force is applied.]]
 <!-- Add more about the biology of this part here
 ===Usage and Biology===