Difference between revisions of "Part:BBa K649101"
 
(26 intermediate revisions by 4 users not shown)
Line 2: Line 2:
 
<partinfo>BBa_K649101 short</partinfo>
 
<partinfo>BBa_K649101 short</partinfo>
  
−
LsrR is the repressor of the lsr operon and itself.  
+
LsrR is the repressor of the lsr operon and itself. Auto Inducer 2(AI-2) is phosphorylated by LsrK, and phospho AI-2 relieves LsrR repression.  
  
−
To confirm LsrR represses lsrA promoter, we constructed BBa_K649105. If you want to see detailed infomation, please click [https://parts.igem.org/Part:BBa_K649105 here.]
+
This part contains <i>lsrR</i> and <i>lsrK</i> gene, downstream of lsrR promoter.  
 +
'''lsrR promoter and <i>lsrR</i> coding gene in this part work correctly, but <i>lsrK</i> has flame shift mutation and does not work properly.'''
  
−
For more information, see our work in Tokyo_Tech 2011 wiki
+
To confirm LsrR represses lsrA promoter, we constructed BBa_K649105.
 +
 
 +
[[Image:lsrRBA.png|thumb|center|500px|Fluorescence intensity is decreased by LsrR repression.<br>This work is done by Hiroki Yoshise.]]
 +
 
 +
We confirmed that LsrR represses lsrA promoter. We measured LsrR repression activity by using a plasmid (BBa_K649105) which have a <i>lsrR</i> gene downstream of lsrA promoter-<i>gfp</i>.By the LsrR repression, the fluorescence intensity decreased 3-fold. This result shows that LsrR successfully repressed lsrA promoter. The working part we created allow the use of AI-2 as a signaling molecule, which is a very powerful tool to build complex Synthetic Biology systems.
 +
 
 +
 
 +
For more information, see our work in [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#6. Tokyo_Tech 2011 wiki].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 14:48, 28 October 2011

PlsrR-lsrR

LsrR is the repressor of the lsr operon and itself. Auto Inducer 2(AI-2) is phosphorylated by LsrK, and phospho AI-2 relieves LsrR repression.

This part contains lsrR and lsrK gene, downstream of lsrR promoter. lsrR promoter and lsrR coding gene in this part work correctly, but lsrK has flame shift mutation and does not work properly.

To confirm LsrR represses lsrA promoter, we constructed BBa_K649105.

Fluorescence intensity is decreased by LsrR repression.
This work is done by Hiroki Yoshise.

We confirmed that LsrR represses lsrA promoter. We measured LsrR repression activity by using a plasmid (BBa_K649105) which have a lsrR gene downstream of lsrA promoter-gfp.By the LsrR repression, the fluorescence intensity decreased 3-fold. This result shows that LsrR successfully repressed lsrA promoter. The working part we created allow the use of AI-2 as a signaling molecule, which is a very powerful tool to build complex Synthetic Biology systems.


For more information, see our work in [http://2011.igem.org/Team:Tokyo_Tech/Projects/RPS-game/assay#6. Tokyo_Tech 2011 wiki].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1725
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1399
  • 1000
    COMPATIBLE WITH RFC[1000]