Difference between revisions of "Part:BBa I723020:Experience"
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<partinfo>BBa_I723020 1</partinfo>
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<I>Sebastian Murmann ETH Zürich 2011</I>
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<I>iGEM ETHZ 2011</I>
 
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We had some troubles to get the part out of the iGEM spring distribution 2011. Although trying for several times, we did not succeed in transforming the plasmid provided in the distribution. Thus we used the plasmid ''pCK04YxylR'' as source for the promoter. We further modified the part by adding a double stop codon to it, resulting in [https://parts.igem.org/Part:BBa_K625002 BBa_K625002]. The second modification was to remove the remaining sequence of ''xylU'', which resulted in the short promoter variant [https://parts.igem.org/Part:BBa_K625002 BBa_K625003]. The characterization and comparison of the new promoters can be found in the characterization section of [https://parts.igem.org/Part:BBa_K625002 BBa_K625002] respectively [https://parts.igem.org/Part:BBa_K625003 BBa_K625003].
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We had trouble getting this part out of the iGEM spring distribution 2011. Although trying for several times, we did not succeed in transforming the plasmid provided in the distribution. Thus we used the plasmid ''pCK04AxylR'' as source for the promoter. We further modified the part by adding a double stop codon to it, to avoid unwanted fusion proteins from emerging. This design resulted in [https://parts.igem.org/Part:BBa_K625002 BBa_K625002]. In a second design, all unnecessary sequences were removed from the promoter, resulting in a minimized version ([https://parts.igem.org/Part:BBa_K625003 BBa_K625003]). The characterization and comparison of the modified promoters can be found in the characterization section of [https://parts.igem.org/Part:BBa_K625002 BBa_K625002] respectively [https://parts.igem.org/Part:BBa_K625003 BBa_K625003].
  
 
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Latest revision as of 16:54, 1 October 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I723020

User Reviews

UNIQ815ce267c23b05e1-partinfo-00000000-QINU

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iGEM ETHZ 2011

We had trouble getting this part out of the iGEM spring distribution 2011. Although trying for several times, we did not succeed in transforming the plasmid provided in the distribution. Thus we used the plasmid pCK04AxylR as source for the promoter. We further modified the part by adding a double stop codon to it, to avoid unwanted fusion proteins from emerging. This design resulted in BBa_K625002. In a second design, all unnecessary sequences were removed from the promoter, resulting in a minimized version (BBa_K625003). The characterization and comparison of the modified promoters can be found in the characterization section of BBa_K625002 respectively BBa_K625003.

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UNIQ815ce267c23b05e1-partinfo-00000002-QINU