Difference between revisions of "Part:BBa K594005:Design"

 
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===Design Notes===
 
===Design Notes===
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Initial experiments showed 3-OH-C14:1-HSL to be an inducer of the rhi genes, but later data showing that rhiI and rhiA could be induced by short-chain AHLs (C6-HSL, C8-HSL, and 3-oxo-C8-HSL) but not 3-OH-C14:1-HSL suggested that the induction was indirect.
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The rhi quorum sensing system show relationships with plasmid transfering. However, real transferring only occurs when these genes are combined with other essential genes like traABC, traI/R, so only rhi quorum sensing could not make the plasmid transfer. They will not arouse biosafety problems.
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===Source===
 
===Source===
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===References===
 
===References===
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1.J. Allan Downie, Gerd Hombrecher*, Qing-Sheng Ma, Celia D. Knight, Brian Wells, and Andrew W.B. Johnston. Cloned Nodulation Genes of Rhizobium leguminosarum Determine Host-Range Specificity. Mol Gen Genet (1983) 190:359-365<br>
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2.Juan E. Gonza´lez and Melanie M. Marketon. 2003, Quorum Sensing in Nitrogen-Fixing Rhizobia, MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, p. 574–592 <br>
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3.Belen Rodelas et al.Analysis of Quorum-Sensing-Dependent Control of Rhizosphere-Expressed (rhi) Genes in Rhizobium leguminosarum bv. viciae, JOURNAL OF BACTERIOLOGY,June 1999, p. 3816–3823.<br>
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4.M. T. CUBO et al. Molecular Characterization and Regulation of the Rhizosphere-Expressed Genes rhiABCR That Can Influence Nodulation by Rhizobium leguminosarum Biovar viciae. JOURNAL OF BACTERIOLOGY, June 1992, p. 4026-4035

Latest revision as of 23:32, 5 October 2011

a luxI homologous AHL synthetase from Rhzobium leguminosarum bv. vicae IMA57


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 394
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The rhi quorum sensing system show relationships with plasmid transfering. However, real transferring only occurs when these genes are combined with other essential genes like traABC, traI/R, so only rhi quorum sensing could not make the plasmid transfer. They will not arouse biosafety problems.

Source

it is from the Rhzobium leguminosarum bv. vicae IMA57 we purchased from the CGMCC(China General Microbiological Culture Collection Center).

References

1.J. Allan Downie, Gerd Hombrecher*, Qing-Sheng Ma, Celia D. Knight, Brian Wells, and Andrew W.B. Johnston. Cloned Nodulation Genes of Rhizobium leguminosarum Determine Host-Range Specificity. Mol Gen Genet (1983) 190:359-365
2.Juan E. Gonza´lez and Melanie M. Marketon. 2003, Quorum Sensing in Nitrogen-Fixing Rhizobia, MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, p. 574–592
3.Belen Rodelas et al.Analysis of Quorum-Sensing-Dependent Control of Rhizosphere-Expressed (rhi) Genes in Rhizobium leguminosarum bv. viciae, JOURNAL OF BACTERIOLOGY,June 1999, p. 3816–3823.
4.M. T. CUBO et al. Molecular Characterization and Regulation of the Rhizosphere-Expressed Genes rhiABCR That Can Influence Nodulation by Rhizobium leguminosarum Biovar viciae. JOURNAL OF BACTERIOLOGY, June 1992, p. 4026-4035