Difference between revisions of "Part:BBa K567001:Design"

(Design Notes)
(Source)
 
Line 10: Line 10:
 
===Source===
 
===Source===
  
LacI- Ptac derived from plasmid pTrc99B. tRNA(Arg) from E.coli ArgW operon.
+
LacI- Ptrc derived from plasmid pTrc99B. tRNA(Arg) from E.coli ArgW operon.
  
 
===References===
 
===References===

Latest revision as of 04:10, 5 October 2011

lacI-Ptrc-tRNA(Arg)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1845
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1845
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1845
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1845
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2026


Design Notes

The correct processing to produce mature tRNA should be ensured.

Source

LacI- Ptrc derived from plasmid pTrc99B. tRNA(Arg) from E.coli ArgW operon.

References